FPR3 Antikörper (N-Term)

Produktnummer ABIN1048609

Produktdetails anti-FPR3 Antikörper

Target: FPR3 (Formyl Peptide Receptor 3 (FPR3))

Bindungsspezifität: N-Term

Reaktivität: Human, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser FPR3 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Marke: IHC-plus™

Spezifität: Human FPR3 / FPRH2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Homologie: Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey (100%) Orangutan (94%) Gibbon (89%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic 18 amino acid peptide from N-terminal extracellular domain of human FPR3 / FPRH2. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey (100%), Orangutan (94%), Gibbon (89%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: ICC, IHC, IHC-P (3 μg/mL)

Kommentare:

Target Species of Antibody: Human

Testdurchführung:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu FPR3

Target: FPR3 (Formyl Peptide Receptor 3 (FPR3))

Andere Bezeichnung: FPR3 / FPRL2 (FPR3 Produkte)

Synonyme: RGD1566044 antikoerper, FML2_HUMAN antikoerper, FMLPY antikoerper, FPRH1 antikoerper, FPRH2 antikoerper, FPRL2 antikoerper, RMLP-R-I antikoerper, ALX antikoerper, Fpr-rs1 antikoerper, Fpr-s1 antikoerper, Fprl1 antikoerper, LXA4-R antikoerper, Lxa4r antikoerper, FMLP-R-II antikoerper, formyl peptide receptor 3 antikoerper, Fpr3 antikoerper, FPR3 antikoerper

Hintergrund: Name/Gene ID: FPR3
Subfamily: Chemoattractant
Family: GPCR

Synonyms: FPR3, FMLP-related receptor II, Formyl peptide receptor 3, Formyl peptide receptor-like 2, FPRL2, FML2_HUMAN, FMLP-R-II, FMLPY, FPRH1, FPRH2, N-formyl peptide receptor 3, N-formyl receptor-like 2, RMLP-R-I

Gen-ID: 2359