FZD10 Antikörper (N-Term)

Produktnummer ABIN1048619

Produktdetails anti-FZD10 Antikörper

Target: FZD10 (Frizzled Family Receptor 10 (FZD10))

Bindungsspezifität: N-Term

Reaktivität: Human, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser FZD10 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human FZD10 / Frizzled 10. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Homologie: Percent identity with other species by BLAST analysis: Human, Monkey (100%) Gibbon, Hamster, Panda, Dog (93%) Marmoset, Mouse, Bovine, Horse, Pig (86%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic 14 amino acid peptide from N-terminal extracellular domain of human FZD10 / Frizzled 10. Percent identity with other species by BLAST analysis: Human, Monkey (100%), Gibbon, Hamster, Panda, Dog (93%), Marmoset, Mouse, Bovine, Horse, Pig (86%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (6 - 16 μg/mL)

Kommentare:

Target Species of Antibody: Human

Testdurchführung:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu FZD10

Target: FZD10 (Frizzled Family Receptor 10 (FZD10))

Andere Bezeichnung: FZD10 / Frizzled 10 (FZD10 Produkte)

Synonyme: CD350 antikoerper, FZ-10 antikoerper, Fz10 antikoerper, FzE7 antikoerper, hFz10 antikoerper, cFz-10 antikoerper, Fz-10 antikoerper, Xfr9 antikoerper, Xfz10 antikoerper, frizzled-10 antikoerper, frizzled10 antikoerper, fz10 antikoerper, fze7 antikoerper, hfz10 antikoerper, fk48e04 antikoerper, fz4 antikoerper, fzb antikoerper, wu:fk48e04 antikoerper, zg04 antikoerper, Xfz10A antikoerper, fzd10a antikoerper, Xfz10B antikoerper, Xfz9 antikoerper, fzd10b antikoerper, fzd9 antikoerper, frizzled class receptor 10 antikoerper, frizzled class receptor 10 L homeolog antikoerper, frizzled class receptor 10 S homeolog antikoerper, FZD10 antikoerper, fzd10 antikoerper, Fzd10 antikoerper, fzd10.L antikoerper, fzd10.S antikoerper

Hintergrund: Name/Gene ID: FZD10
Subfamily: Frizzled
Family: GPCR

Synonyms: FZD10, CD350 antigen, CD350, FZ-10, Frizzled homolog 10, HFz10, Frizzled 10, Frizzled family receptor 10, Frizzled-10, FzE7, Fz10

Gen-ID: 11211

Pathways: WNT Signalweg