FZD8 Antikörper (Extracellular Domain)

Produktnummer ABIN1048630

Produktdetails anti-FZD8 Antikörper

Target: FZD8 (Frizzled Family Receptor 8 (FZD8))

Bindungsspezifität: Extracellular Domain

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund, Affe, Kaninchen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser FZD8 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human FZD8 / Frizzled 8. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except INTS6 (50 % ).

Homologie: Percent identity with other species by BLAST analysis: Human, Marmoset, Mouse, Rat, Elephant, Panda, Bovine, Dog, Rabbit, Opossum (100%) Lizard (94%) Monkey (81%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic 16 amino acid peptide from 3rd extracellular domain of human FZD8 / Frizzled 8. Percent identity with other species by BLAST analysis: Human, Marmoset, Mouse, Rat, Elephant, Panda, Bovine, Dog, Rabbit, Opossum (100%), Lizard (94%), Monkey (81%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (8 - 20 μg/mL)

Kommentare:

Target Species of Antibody: Human

Testdurchführung:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu FZD8

Target: FZD8 (Frizzled Family Receptor 8 (FZD8))

Andere Bezeichnung: FZD8 / Frizzled 8 (FZD8 Produkte)

Synonyme: FZ-8 antikoerper, hFZ8 antikoerper, FZD8 antikoerper, Fz-8 antikoerper, frizzled8 antikoerper, xfz8 antikoerper, Xfz8 antikoerper, frizzled-8 antikoerper, hfz8 antikoerper, Fz8 antikoerper, fzd8 antikoerper, fz5 antikoerper, fz8a antikoerper, fzc antikoerper, wu:fd02c05 antikoerper, zg05 antikoerper, ZG06 antikoerper, Zfz8b antikoerper, fz6 antikoerper, fz8b antikoerper, fzA antikoerper, ik:tdsubc_1e1 antikoerper, xx:tdsubc_1e1 antikoerper, zfzA antikoerper, frizzled class receptor 8 antikoerper, frizzled class receptor 8 S homeolog antikoerper, frizzled class receptor 8 L homeolog antikoerper, frizzled class receptor 8a antikoerper, frizzled class receptor 8b antikoerper, FZD8 antikoerper, fzd8.S antikoerper, fzd8 antikoerper, Fzd8 antikoerper, fzd8.L antikoerper, fzd8a antikoerper, fzd8b antikoerper

Hintergrund: Name/Gene ID: FZD8
Subfamily: Frizzled
Family: GPCR

Synonyms: FZD8, Frizzled family receptor 8, Frizzled homolog 8, FZ-8, Fz8, Frizzled 8, Frizzled-8, HFZ8

Gen-ID: 8325

Pathways: WNT Signalweg