FZD9 Antikörper (Extracellular Domain)

Produktnummer ABIN1048632

Produktdetails anti-FZD9 Antikörper

Target: FZD9 (Frizzled Family Receptor 9 (FZD9))

Bindungsspezifität: Extracellular Domain

Reaktivität: Human, Maus, Ratte, Hund, Rind (Kuh), Kaninchen, Fledermaus, Hamster

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser FZD9 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human FZD9 / Frizzled 9. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Homologie: Percent identity with other species by BLAST analysis: Human, Gibbon, Mouse, Rat, Hamster, Panda, Dog, Bovine, Bat, Rabbit, Opossum (100%) Platypus (86%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic 14 amino acid peptide from 1st extracellular domain of human FZD9 / Frizzled 9. Percent identity with other species by BLAST analysis: Human, Gibbon, Mouse, Rat, Hamster, Panda, Dog, Bovine, Bat, Rabbit, Opossum (100%), Platypus (86%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (10 μg/mL)

Kommentare:

Target Species of Antibody: Human

Testdurchführung:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu FZD9

Target: FZD9 (Frizzled Family Receptor 9 (FZD9))

Andere Bezeichnung: FZD9 / Frizzled 9 (FZD9 Produkte)

Synonyme: CD349 antikoerper, FZD3 antikoerper, mfz9 antikoerper, Fz-9 antikoerper, cFz-9 antikoerper, fz11 antikoerper, fzd9 antikoerper, fzx antikoerper, hm:zehl0603 antikoerper, zehl0603 antikoerper, zg11 antikoerper, frizzled class receptor 9 antikoerper, frizzled class receptor 9a antikoerper, frizzled class receptor 9b antikoerper, FZD9 antikoerper, Fzd9 antikoerper, fzd9a antikoerper, fzd9b antikoerper

Hintergrund: Name/Gene ID: FZD9
Subfamily: Frizzled
Family: GPCR

Synonyms: FZD9, CD349, Frizzled homolog 9, Fz-9, Frizzled family receptor 9, Frizzled-9, Frizzled 9, Fz9, HFz9, CD349 antigen, Frizzled homolog fzd3, FzE6

Gen-ID: 8326

Pathways: WNT Signalweg