GLP1R Antikörper (N-Term)
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- Target Alle GLP1R Antikörper anzeigen
- GLP1R (Glucagon-Like Peptide 1 Receptor (GLP1R))
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Bindungsspezifität
- N-Term
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Reaktivität
- Human, Maus
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser GLP1R Antikörper ist unkonjugiert
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Applikation
- Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Marke
- IHC-plus™
- Spezifität
- Human GLP1R. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- Aufreinigung
- Immunoaffinity purified
- Immunogen
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Synthetic 16 amino acid peptide from N-terminal extracellular domain of human GLP1R. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%), Marmoset, Mouse, Rat, Sheep, Bovine, Hamster, Elephant, Rabbit, Pig (94%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product GLP1R Primärantikörper
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- Applikationshinweise
- Approved: IF, IHC, IHC-Fr, IHC-P (3 - 4 μg/mL)
- Kommentare
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Target Species of Antibody: Human
- Testdurchführung
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- Lot specific
- Buffer
- PBS, less than 0.1 % sodium azide.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- Haltbarkeit
- 12 months
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- Target
- GLP1R (Glucagon-Like Peptide 1 Receptor (GLP1R))
- Andere Bezeichnung
- GLP1R/GLP-1R/GLP-1 Receptor (GLP1R Produkte)
- Synonyme
- GLP1R antikoerper, LOC100228311 antikoerper, GLP-1R antikoerper, GLP1Rc antikoerper, Glip antikoerper, RATGL1RCP antikoerper, glucagon like peptide 1 receptor antikoerper, glucagon-like peptide 1 receptor antikoerper, GLP1R antikoerper, Glp1r antikoerper
- Hintergrund
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Name/Gene ID: GLP1R
Subfamily: Glucagon
Family: GPCR
Synonyms: GLP1R, GLP-1-R, GLP-1 receptor, GLP-1R, GLP-I receptor, Glp1 receptor - Gen-ID
- 2740
- Pathways
- Positive Regulation of Peptide Hormone Secretion, Hormone Transport, cAMP Metabolic Process, Feeding Behaviour
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