GPR109B Antikörper (Cytoplasmic Domain)

Produktnummer ABIN1048689

Produktdetails anti-GPR109B Antikörper

Target: GPR109B (HCAR3) (Hydroxycarboxylic Acid Receptor 3 (HCAR3))

Bindungsspezifität: Cytoplasmic Domain

Reaktivität: Human, Hund, Hamster

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GPR109B Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Marke: IHC-plus™

Spezifität: Human GPR109B / HM74. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except HCAR2 (100 % ), GPR81 (50 %).

Homologie: Percent identity with other species by BLAST analysis: Human, Gorilla, Dog, Hamster, Panda (100%) Mouse, Horse, Guinea pig (95%) Rat (90%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic 20 amino acid peptide from 3rd cytoplasmic domain of human GPR109B / HM74. Percent identity with other species by BLAST analysis: Human, Gorilla, Dog, Hamster, Panda (100%), Mouse, Horse, Guinea pig (95%), Rat (90%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: ICC, IHC, IHC-P (19 - 38 μg/mL)

Kommentare:

Target Species of Antibody: Human

Testdurchführung:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu GPR109B

Target: GPR109B (HCAR3) (Hydroxycarboxylic Acid Receptor 3 (HCAR3))

Andere Bezeichnung: HCAR3 / GPR109B / HM74 (HCAR3 Produkte)

Synonyme: GPR109B antikoerper, HCA3 antikoerper, HM74 antikoerper, PUMAG antikoerper, Puma-g antikoerper, NIACR2 antikoerper, hm74 antikoerper, niacr2 antikoerper, hydroxycarboxylic acid receptor 3 antikoerper, HCAR3 antikoerper, hcar3 antikoerper

Hintergrund: Name/Gene ID: HCAR3
Subfamily: Nicotinic Acid
Family: GPCR

Synonyms: HCAR3, GPR109B, GTP-binding protein, NIACR2, HM74, HM74b, Nicotinic acid receptor 2, Putative chemokine receptor, Niacin receptor 2, Puma-g, HCA3, PUMAG

Gen-ID: 8843