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GPR137B Antikörper (C-Term)

GPR137B Reaktivität: Human, Maus, Ratte, Pferd, Affe, Schwein, Kaninchen IHC (p), IHC, ICC Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN1048719
  • Target Alle GPR137B Produkte
    GPR137B (G Protein-Coupled Receptor 137B (GPR137B))
    Bindungsspezifität
    • 7
    • 1
    • 1
    • 1
    C-Term
    Reaktivität
    • 19
    • 11
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    Human, Maus, Ratte, Pferd, Affe, Schwein, Kaninchen
    Wirt
    • 19
    Kaninchen
    Klonalität
    • 19
    Polyklonal
    Konjugat
    • 12
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser GPR137B Antikörper ist unkonjugiert
    Applikation
    • 7
    • 6
    • 5
    • 3
    • 3
    • 1
    • 1
    Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
    Marke
    IHC-plus™
    Spezifität
    Human GPR137B / TM7SF1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
    Homologie
    Percent identity with other species by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Mouse, Rat, Panda, Horse, Rabbit, Pig, Opossum (100%) Hamster, Elephant, Bovine (94%) Goat, Chicken, Lizard (88%) Dog, Bat, Turkey (81%).
    Aufreinigung
    Immunoaffinity purified
    Immunogen
    Synthetic 16 amino acid peptide from C-terminus of human GPR137B / TM7SF1. Percent identity with other species by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Mouse, Rat, Panda, Horse, Rabbit, Pig, Opossum (100%), Hamster, Elephant, Bovine (94%), Goat, Chicken, Lizard (88%), Dog, Bat, Turkey (81%).

    Type of Immunogen: Synthetic peptide
  • Applikationshinweise
    Approved: ICC, IHC, IHC-P (5 - 12 μg/mL)
    Kommentare

    Target Species of Antibody: Human

    Testdurchführung

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    Lot specific
    Buffer
    PBS, less than 0.1 % sodium azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    Haltbarkeit
    12 months
  • Target
    GPR137B (G Protein-Coupled Receptor 137B (GPR137B))
    Andere Bezeichnung
    GPR137B (GPR137B Produkte)
    Synonyme
    TM7SF1 antikoerper, AI788917 antikoerper, AU016435 antikoerper, AW546472 antikoerper, C730041E01 antikoerper, C80550 antikoerper, C80741 antikoerper, D13Abb1e antikoerper, Tm7sf1 antikoerper, GPR137B antikoerper, gpr137ba antikoerper, tm7sf1a antikoerper, wu:fi39g02 antikoerper, zgc:101671 antikoerper, G protein-coupled receptor 137B antikoerper, G protein-coupled receptor 137B L homeolog antikoerper, G protein-coupled receptor 137c antikoerper, GPR137B antikoerper, gpr137b.L antikoerper, Gpr137b antikoerper, gpr137b antikoerper, gpr137c antikoerper
    Hintergrund
    Name/Gene ID: GPR137B
    Subfamily: Orphan-U
    Family: GPCR

    Synonyms: GPR137B, TM7SF1
    Gen-ID
    7107
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