GPR162 Antikörper (Transmembrane Domain)
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- Target Alle GPR162 Antikörper anzeigen
- GPR162 (G Protein-Coupled Receptor 162 (GPR162))
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Bindungsspezifität
- Transmembrane Domain
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Reaktivität
- Human, Affe
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser GPR162 Antikörper ist unkonjugiert
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Applikation
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Marke
- IHC-plus™
- Spezifität
- Human GPR162. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- Homologie
- Percent identity with other species by BLAST analysis: Human, Monkey (100%) Gibbon, Rat, Dog, Pig (95%) Mouse, Bovine, Hamster, Panda, Horse (89%) Bat, Elephant (84%).
- Aufreinigung
- Immunoaffinity purified
- Immunogen
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Synthetic 19 amino acid peptide from 6th transmembrane domain of human GPR162. Percent identity with other species by BLAST analysis: Human, Monkey (100%), Gibbon, Rat, Dog, Pig (95%), Mouse, Bovine, Hamster, Panda, Horse (89%), Bat, Elephant (84%).
Type of Immunogen: Synthetic peptide
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- Applikationshinweise
- Approved: IHC, IHC-P (6 μg/mL)
- Kommentare
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Target Species of Antibody: Human
- Testdurchführung
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- Lot specific
- Buffer
- PBS, less than 0.1 % sodium azide.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- Haltbarkeit
- 12 months
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- Target
- GPR162 (G Protein-Coupled Receptor 162 (GPR162))
- Andere Bezeichnung
- GPR162 (GPR162 Produkte)
- Synonyme
- Grca antikoerper, A-2 antikoerper, GRCA antikoerper, BC031437 antikoerper, G protein-coupled receptor 162 antikoerper, Gpr162 antikoerper, GPR162 antikoerper
- Hintergrund
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Name/Gene ID: GPR162
Subfamily: Orphan-U
Family: GPCR
Synonyms: GPR162, A-2, Gene rich cluster, A, G protein-coupled receptor 162, GRCA - Gen-ID
- 27239
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