GPR68 Antikörper (C-Term)

Produktnummer ABIN1048863

Produktdetails anti-GPR68 Antikörper

Target: GPR68 (G Protein-Coupled Receptor 68 (GPR68))

Bindungsspezifität: C-Term

Reaktivität: Human, Maus, Ratte, Pferd, Rind (Kuh), Kaninchen, Affe, Schwein, Hamster

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GPR68 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunocytochemistry (ICC)

Marke: IHC-plus™

Spezifität: Human GPR68. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Homologie: Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Bovine, Hamster, Elephant, Horse, Rabbit, Pig (100%) Dog, Bat, Panda, Platypus (88%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic 16 amino acid peptide from C-terminus of human GPR68. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Bovine, Hamster, Elephant, Horse, Rabbit, Pig (100%), Dog, Bat, Panda, Platypus (88%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: ICC, IF, IHC, IHC-P (10 - 20 μg/mL)

Not recommended for: IHC-P

Kommentare:

Target Species of Antibody: Human

Testdurchführung:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-GPR68 Antikörper (ABIN1048863)

Komarova, Pereverzev, Shum, Sims, Dixon: "Convergent signaling by acidosis and receptor activator of NF-kappaB ligand (RANKL) on the calcium/calcineurin/NFAT pathway in osteoclasts." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 7, pp. 2643-8, (2005) (PubMed).

Details zu GPR68

Target: GPR68 (G Protein-Coupled Receptor 68 (GPR68))

Andere Bezeichnung: GPR68 / OGR1 (GPR68 Produkte)

Synonyme: GPR68 antikoerper, GPR12A antikoerper, OGR1 antikoerper, BB131428 antikoerper, Ogr1 antikoerper, G protein-coupled receptor 68 antikoerper, GPR68 antikoerper, LOC100348269 antikoerper, Gpr68 antikoerper

Hintergrund: Name/Gene ID: GPR68
Subfamily: Lysophospholipid/Lysosphingolipid
Family: GPCR

Synonyms: GPR68, Brgrb, G protein-coupled receptor 68, G-protein coupled receptor 68, OGR1, Brgr1, GPR12A, OGR-1

Gen-ID: 8111