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Metabotropic Glutamate Receptor 3 Antikörper (N-Term)

GRM3 Reaktivität: Human, Rind (Kuh), Pferd, Affe, Schwein WB, IHC, ICC, IHC (p) Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN1048932
  • Target Alle Metabotropic Glutamate Receptor 3 (GRM3) Antikörper anzeigen
    Metabotropic Glutamate Receptor 3 (GRM3) (Glutamate Receptor, Metabotropic 3 (GRM3))
    Bindungsspezifität
    • 16
    • 9
    • 5
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term
    Reaktivität
    • 36
    • 29
    • 12
    • 7
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    Human, Rind (Kuh), Pferd, Affe, Schwein
    Wirt
    • 50
    • 2
    • 1
    Kaninchen
    Klonalität
    • 50
    • 3
    Polyklonal
    Konjugat
    • 21
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser Metabotropic Glutamate Receptor 3 Antikörper ist unkonjugiert
    Applikation
    • 41
    • 19
    • 16
    • 9
    • 7
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Marke
    IHC-plus™
    Spezifität
    Human GRM3 / MGLUR3. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
    Homologie
    Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Elephant, Panda, Bovine, Horse, Pig, Opossum (100%) Mouse, Rat, Rabbit (94%) Turkey, Chicken, Xenopus (81%).
    Aufreinigung
    Immunoaffinity purified
    Immunogen
    Synthetic 16 amino acid peptide from N-terminal extracellular domain of human GRM3 / MGLUR3. Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Elephant, Panda, Bovine, Horse, Pig, Opossum (100%), Mouse, Rat, Rabbit (94%), Turkey, Chicken, Xenopus (81%).

    Type of Immunogen: Synthetic peptide
    Top Product
    Discover our top product GRM3 Primärantikörper
  • Applikationshinweise
    Approved: ICC, IHC, IHC-P (5 - 10 μg/mL), WB
    Kommentare

    Target Species of Antibody: Human

    Testdurchführung

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    Lot specific
    Buffer
    PBS, less than 0.1 % sodium azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    Haltbarkeit
    12 months
  • Target
    Metabotropic Glutamate Receptor 3 (GRM3) (Glutamate Receptor, Metabotropic 3 (GRM3))
    Andere Bezeichnung
    GRM3 / MGLUR3 (GRM3 Produkte)
    Synonyme
    GLUR3 antikoerper, GPRC1C antikoerper, MGLUR3 antikoerper, mGlu3 antikoerper, 0710001G23Rik antikoerper, Gprc1c antikoerper, mGluR3 antikoerper, glutamate metabotropic receptor 3 antikoerper, glutamate receptor, metabotropic 3 antikoerper, GRM3 antikoerper, Grm3 antikoerper
    Hintergrund
    Name/Gene ID: GRM3
    Subfamily: Metabotropic glutamate
    Family: GPCR

    Synonyms: GRM3, GPRC1C, GLUR3, MGLUR3, MGlu3
    Gen-ID
    2913
    Pathways
    cAMP Metabolic Process, Synaptic Membrane
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