Metabotropic Glutamate Receptor 6 Antikörper (N-Term)
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- Target Alle Metabotropic Glutamate Receptor 6 (GRM6) Antikörper anzeigen
- Metabotropic Glutamate Receptor 6 (GRM6) (Glutamate Receptor, Metabotropic 6 (GRM6))
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Bindungsspezifität
- N-Term
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Reaktivität
- Human, Maus, Ratte, Hund, Kaninchen, Rind (Kuh), Affe, Schwein, Fledermaus, Hamster
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser Metabotropic Glutamate Receptor 6 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Marke
- IHC-plus™
- Spezifität
- Human GRM6 / MGLUR6. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except GRM7 (73 % ), GRM4 (67 %).
- Homologie
- Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Bovine, Dog, Bat, Rabbit, Pig, Pufferfish (100%) Panda, Horse, Opossum, Lizard, Zebrafish (93%) Platypus, Seabass, Catfish, Stickleback (87%) Xenopus (80%).
- Aufreinigung
- Immunoaffinity purified
- Immunogen
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Synthetic 15 amino acid peptide from N-terminal extracellular domain of human GRM6 / MGLUR6. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Bovine, Dog, Bat, Rabbit, Pig, Pufferfish (100%), Panda, Horse, Opossum, Lizard, Zebrafish (93%), Platypus, Seabass, Catfish, Stickleback (87%), Xenopus (80%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product GRM6 Primärantikörper
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- Applikationshinweise
- Approved: IHC, IHC-P (12 μg/mL), WB
- Kommentare
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Target Species of Antibody: Human
- Testdurchführung
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- Lot specific
- Buffer
- PBS, less than 0.1 % sodium azide.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- Haltbarkeit
- 12 months
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- Target
- Metabotropic Glutamate Receptor 6 (GRM6) (Glutamate Receptor, Metabotropic 6 (GRM6))
- Andere Bezeichnung
- GRM6 / MGLUR6 (GRM6 Produkte)
- Synonyme
- mGluR6 antikoerper, CSNB1B antikoerper, GPRC1F antikoerper, MGLUR6 antikoerper, mGlu6 antikoerper, GRM6 antikoerper, BC021919 antikoerper, Gm3 antikoerper, Gprc1f antikoerper, nerg1 antikoerper, nob2 antikoerper, nob3 antikoerper, nob4 antikoerper, si:dkey-52h10.3 antikoerper, cb496 antikoerper, grma antikoerper, zK13A21.16 antikoerper, zgc:158329 antikoerper, glutamate metabotropic receptor 6 antikoerper, glutamate receptor, metabotropic 6 antikoerper, glutamate receptor, metabotropic 6a antikoerper, glutamate receptor, metabotropic 6b antikoerper, Grm6 antikoerper, GRM6 antikoerper, grm6a antikoerper, grm6b antikoerper
- Hintergrund
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Name/Gene ID: GRM6
Subfamily: Metabotropic glutamate
Family: GPCR
Synonyms: GRM6, CSNB1B, GPRC1F, MGlu6, MGLUR6 - Gen-ID
- 2916
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