Serotonin Receptor 6 Antikörper (C-Term)

Produktnummer ABIN1048968

Produktdetails anti-Serotonin Receptor 6 Antikörper

Target: Serotonin Receptor 6 (HTR6)

Bindungsspezifität: C-Term

Reaktivität: Human, Ratte, Hund, Hamster, Pferd, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Serotonin Receptor 6 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)

Marke: IHC-plus™

Spezifität: Human HTR6 / 5-HT36. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Homologie: Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Rat, Hamster, Panda, Dog, Horse (100%) Mouse, Elephant, Bovine, Bat, Rabbit (94%) Stickleback, Pufferfish (88%) Turkey, Chicken, Xenopus, Zebrafish (82%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic 17 amino acid peptide from C-terminus of human HTR6 / 5-HT36. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Rat, Hamster, Panda, Dog, Horse (100%), Mouse, Elephant, Bovine, Bat, Rabbit (94%), Stickleback, Pufferfish (88%), Turkey, Chicken, Xenopus, Zebrafish (82%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (10 - 20 μg/mL)

Kommentare:

Target Species of Antibody: Human

Testdurchführung:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu Serotonin Receptor 6

Target: Serotonin Receptor 6 (HTR6)

Andere Bezeichnung: HTR6 / 5-HT6 Receptor (HTR6 Produkte)

Synonyme: 5-HT6 antikoerper, 5-HT6R antikoerper, 5-hydroxytryptamine (serotonin) receptor 6 antikoerper, 5-hydroxytryptamine receptor 6 antikoerper, Htr6 antikoerper, HTR6 antikoerper

Hintergrund: Name/Gene ID: HTR6
Subfamily: Serotonin
Family: GPCR

Synonyms: HTR6, 5-HT6, 5-HT6R, 5-HT-6, 5-hydroxytryptamine receptor 6, 5-HT6 receptor, 5-HT6 serotonin receptor, 5-hydroxytryptamine 6 receptor, Serotonin 6 receptor, Serotonin receptor 6, Serotonin 5-HT-6 receptor, Serotonin type 6 receptor

Gen-ID: 3362

Pathways: JAK-STAT Signalweg