Leukotriene B4 Receptor/BLT Antikörper (Cytoplasmic Domain)

Produktnummer ABIN1049000

Produktdetails anti-Leukotriene B4 Receptor/BLT Antikörper

Target: Leukotriene B4 Receptor/BLT (LTB4R) (Leukotriene B4 Receptor (LTB4R))

Bindungsspezifität: Cytoplasmic Domain

Reaktivität: Human, Maus, Ratte, Affe, Kaninchen, Hamster, Pferd

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Leukotriene B4 Receptor/BLT Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human Leukotriene B4 Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Homologie: Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Horse, Rabbit (100%) Bovine (88%) Opossum, Platypus (81%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic 16 amino acid peptide from 3rd cytoplasmic domain of human Leukotriene B4 Receptor. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Horse, Rabbit (100%), Bovine (88%), Opossum, Platypus (81%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-Fr, IHC-P (5 - 10 μg/mL)

Kommentare:

Target Species of Antibody: Human

Testdurchführung:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-Leukotriene B4 Receptor/BLT Antikörper (ABIN1049000)

Bäck, Bu, Bränström, Sheikine, Yan, Hansson: "Leukotriene B4 signaling through NF-kappaB-dependent BLT1 receptors on vascular smooth muscle cells in atherosclerosis and intimal hyperplasia." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 48, pp. 17501-6, (2005) (PubMed).

Details zu Leukotriene B4 Receptor/BLT

Target: Leukotriene B4 Receptor/BLT (LTB4R) (Leukotriene B4 Receptor (LTB4R))

Andere Bezeichnung: Leukotriene B4 Receptor / BLT1 (LTB4R Produkte)

Synonyme: BLT1 antikoerper, BLTR antikoerper, CMKRL1 antikoerper, GPR16 antikoerper, LTB4R1 antikoerper, LTBR1 antikoerper, P2RY7 antikoerper, P2Y7 antikoerper, Ltb4r1 antikoerper, LTB4R antikoerper, lt4r1 antikoerper, Ltb4r antikoerper, mBLTR antikoerper, leukotriene B4 receptor antikoerper, leukotriene B4 receptor-like antikoerper, Leukotriene B4 receptor 1 antikoerper, leukotriene B4 receptor 1 antikoerper, LTB4R antikoerper, Ltb4r antikoerper, LOC415617 antikoerper, lt4r1 antikoerper, Ltb4r1 antikoerper

Hintergrund: Name/Gene ID: LTB4R
Subfamily: Chemoattractant
Family: GPCR

Synonyms: LTB4R, BLT, BLT1, BLTR, Blt-1, Chemokine receptor-like 1, G protein-coupled receptor 16, GPR16, Leukotriene B4 receptor, Leukotriene B4 receptor 1, G-protein coupled receptor 16, LTBR1, p2Y purinoceptor 7, p2Y7, LTB4-R 1, p2RY7, CMKRL1, LTB4-R1, LTB4R1

Gen-ID: 1241