MAP4K6 Antikörper (Internal Region)

Produktnummer ABIN1049061

Produktdetails anti-MAP4K6 Antikörper

Target: MAP4K6 (MINK1) (Misshapen-Like Kinase 1 (MINK1))

Bindungsspezifität: Internal Region

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Schwein, Pferd, Fledermaus, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MAP4K6 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human MINK1 / MAP4K6. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Homologie: Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Bovine, Bat, Horse, Pig (100%) Opossum (94%) Xenopus (83%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic 18 amino acid peptide from internal region of human MINK1 / MAP4K6. Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Bovine, Bat, Horse, Pig (100%), Opossum (94%), Xenopus (83%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (7 μg/mL)

Kommentare:

Target Species of Antibody: Human

Testdurchführung:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu MAP4K6

Target: MAP4K6 (MINK1) (Misshapen-Like Kinase 1 (MINK1))

Andere Bezeichnung: MINK1 / MINK (MINK1 Produkte)

Synonyme: MINK1 antikoerper, MINK antikoerper, B55 antikoerper, MAP4K6 antikoerper, YSK2 antikoerper, ZC3 antikoerper, hMINK antikoerper, hMINKbeta antikoerper, MEKKK 6 antikoerper, Map4k6 antikoerper, RP23-122P1.6 antikoerper, Ysk2 antikoerper, misshapen like kinase 1 antikoerper, misshapen-like kinase 1 antikoerper, misshapen-like kinase 1 (zebrafish) antikoerper, MINK1 antikoerper, Mink1 antikoerper

Hintergrund: Name/Gene ID: MINK1
Subfamily: MSN
Family: Protein Kinase

Synonyms: MINK1, HMINKbeta, GCK family kinase MiNK, MAP4K6, Misshapen/NIK-related kinase, MinK, Mink 1, Misshapen/NIKs-related kinase, HMINK, MEKKK 6, Mink 2, B55, ZC3, MEK kinase kinase 6, Misshapen-like kinase 1, YSK2

Gen-ID: 50488

Pathways: Synaptic Membrane