MRGPRX1 Antikörper (N-Term)
-
- Target Alle MRGPRX1 Antikörper anzeigen
- MRGPRX1 (MAS-Related GPR, Member X1 (MRGPRX1))
-
Bindungsspezifität
- N-Term
-
Reaktivität
- Human, Affe
-
Wirt
- Kaninchen
-
Klonalität
- Polyklonal
-
Konjugat
- Dieser MRGPRX1 Antikörper ist unkonjugiert
-
Applikation
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
- Marke
- IHC-plus™
- Spezifität
- Human MRGPRX1 / MRGX1. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except MRGPRX3 (71 % ).
- Homologie
- Percent identity with other species by BLAST analysis: Human, Monkey (100%) Gibbon (93%).
- Aufreinigung
- Immunoaffinity purified
- Immunogen
-
Synthetic 14 amino acid peptide from N-terminal extracellular domain of human MRGPRX1 / MRGX1. Percent identity with other species by BLAST analysis: Human, Monkey (100%), Gibbon (93%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product MRGPRX1 Primärantikörper
-
-
- Applikationshinweise
- Approved: ICC, IHC, IHC-P (12 μg/mL)
- Kommentare
-
Target Species of Antibody: Human
- Testdurchführung
-
The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
- Format
- Liquid
- Konzentration
- Lot specific
- Buffer
- PBS, less than 0.1 % sodium azide.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- Haltbarkeit
- 12 months
-
- Target
- MRGPRX1 (MAS-Related GPR, Member X1 (MRGPRX1))
- Andere Bezeichnung
- MRGX1 / MRGPRX1 (MRGPRX1 Produkte)
- Synonyme
- GPCR antikoerper, MRGX1 antikoerper, SNSR4 antikoerper, MrgC11 antikoerper, Mrgprc11 antikoerper, Snsr1 antikoerper, MAS related GPR family member X1 antikoerper, MAS-related GPR, member X1 antikoerper, MRGPRX1 antikoerper, Mrgprx1 antikoerper
- Hintergrund
-
Name/Gene ID: MRGPRX1
Subfamily: Opioid
Family: GPCR
Synonyms: MRGPRX1, MRGX1, SNSR4, SNSR3, MAS-related GPR, member X1 - Gen-ID
- 259249
-