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NEK9 Antikörper

NEK9 Reaktivität: Human, Pferd, Kaninchen, Schwein, Affe, Hamster IHC, IHC (p) Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN1049094
  • Target Alle NEK9 Antikörper anzeigen
    NEK9 (NIMA (Never in Mitosis Gene A)- Related Kinase 9 (NEK9))
    Reaktivität
    • 80
    • 52
    • 28
    • 5
    • 5
    • 5
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    Human, Pferd, Kaninchen, Schwein, Affe, Hamster
    Wirt
    • 75
    • 5
    Kaninchen
    Klonalität
    • 78
    • 2
    Polyklonal
    Konjugat
    • 35
    • 7
    • 7
    • 6
    • 4
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser NEK9 Antikörper ist unkonjugiert
    Applikation
    • 65
    • 40
    • 20
    • 13
    • 12
    • 7
    • 6
    • 2
    • 1
    • 1
    • 1
    • 1
    Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Marke
    IHC-plus™
    Spezifität
    Human NEK9. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
    Homologie
    Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Hamster, Elephant, Panda, Horse, Rabbit, Pig (100%) Mouse, Rat, Dog, Bovine, Bat, Opossum (93%) Platypus (87%) Turkey, Chicken (80%).
    Aufreinigung
    Immunoaffinity purified
    Immunogen
    Synthetic 15 amino acid peptide from Pro/Ser/Thr rich domain of human NEK9. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Hamster, Elephant, Panda, Horse, Rabbit, Pig (100%), Mouse, Rat, Dog, Bovine, Bat, Opossum (93%), Platypus (87%), Turkey, Chicken (80%).

    Type of Immunogen: Synthetic peptide
    Top Product
    Discover our top product NEK9 Primärantikörper
  • Applikationshinweise
    Approved: IHC, IHC-P (10 μg/mL)

    Usage: Proteinase K treatment is required for antigen retrieval.
    Kommentare

    Target Species of Antibody: Human

    Testdurchführung

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    Lot specific
    Buffer
    PBS, less than 0.1 % sodium azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    Haltbarkeit
    12 months
  • Target
    NEK9 (NIMA (Never in Mitosis Gene A)- Related Kinase 9 (NEK9))
    Andere Bezeichnung
    NEK9 (NEK9 Produkte)
    Synonyme
    NERCC antikoerper, NERCC1 antikoerper, Nek8 antikoerper, NEK9 antikoerper, nercc antikoerper, nercc1 antikoerper, C130021H08Rik antikoerper, NIMA related kinase 9 antikoerper, NIMA-related kinase 9 antikoerper, NIMA related kinase 8 antikoerper, NIMA (never in mitosis gene a)-related expressed kinase 9 antikoerper, NIMA-related kinase 9 L homeolog antikoerper, NEK9 antikoerper, nek9 antikoerper, NEK8 antikoerper, Nek9 antikoerper, nek9.L antikoerper
    Hintergrund
    Name/Gene ID: NEK9
    Subfamily: NEK
    Family: Protein Kinase

    Synonyms: NEK9, KIAA1995, NERCC, NERCC1, Nercc1 kinase, NIMA-related kinase 8, NIMA-related kinase Nek8, NIMA-related kinase 9, NimA-related protein kinase 9
    Gen-ID
    91754
    Pathways
    SARS-CoV-2 Protein Interaktom, Phosphorylierungen bei SARS-CoV-2 Infektion
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