NEK9 Antikörper
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- Target Alle NEK9 Antikörper anzeigen
- NEK9 (NIMA (Never in Mitosis Gene A)- Related Kinase 9 (NEK9))
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Reaktivität
- Human, Pferd, Kaninchen, Schwein, Affe, Hamster
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser NEK9 Antikörper ist unkonjugiert
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Applikation
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Marke
- IHC-plus™
- Spezifität
- Human NEK9. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- Homologie
- Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Hamster, Elephant, Panda, Horse, Rabbit, Pig (100%) Mouse, Rat, Dog, Bovine, Bat, Opossum (93%) Platypus (87%) Turkey, Chicken (80%).
- Aufreinigung
- Immunoaffinity purified
- Immunogen
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Synthetic 15 amino acid peptide from Pro/Ser/Thr rich domain of human NEK9. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Hamster, Elephant, Panda, Horse, Rabbit, Pig (100%), Mouse, Rat, Dog, Bovine, Bat, Opossum (93%), Platypus (87%), Turkey, Chicken (80%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product NEK9 Primärantikörper
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- Applikationshinweise
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Approved: IHC, IHC-P (10 μg/mL)
Usage: Proteinase K treatment is required for antigen retrieval. - Kommentare
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Target Species of Antibody: Human
- Testdurchführung
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- Lot specific
- Buffer
- PBS, less than 0.1 % sodium azide.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- Haltbarkeit
- 12 months
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- Target
- NEK9 (NIMA (Never in Mitosis Gene A)- Related Kinase 9 (NEK9))
- Andere Bezeichnung
- NEK9 (NEK9 Produkte)
- Synonyme
- NERCC antikoerper, NERCC1 antikoerper, Nek8 antikoerper, NEK9 antikoerper, nercc antikoerper, nercc1 antikoerper, C130021H08Rik antikoerper, NIMA related kinase 9 antikoerper, NIMA-related kinase 9 antikoerper, NIMA related kinase 8 antikoerper, NIMA (never in mitosis gene a)-related expressed kinase 9 antikoerper, NIMA-related kinase 9 L homeolog antikoerper, NEK9 antikoerper, nek9 antikoerper, NEK8 antikoerper, Nek9 antikoerper, nek9.L antikoerper
- Hintergrund
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Name/Gene ID: NEK9
Subfamily: NEK
Family: Protein Kinase
Synonyms: NEK9, KIAA1995, NERCC, NERCC1, Nercc1 kinase, NIMA-related kinase 8, NIMA-related kinase Nek8, NIMA-related kinase 9, NimA-related protein kinase 9 - Gen-ID
- 91754
- Pathways
- SARS-CoV-2 Protein Interaktom, Phosphorylierungen bei SARS-CoV-2 Infektion
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