Prokineticin Receptor 2 Antikörper (N-Term)

Produktnummer ABIN1049244

Produktdetails anti-Prokineticin Receptor 2 Antikörper

Target: Prokineticin Receptor 2 (PROKR2)

Bindungsspezifität: N-Term

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Prokineticin Receptor 2 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human PROKR2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Homologie: Percent identity with other species by BLAST analysis: Human, Gibbon (100%) Monkey, Marmoset (93%) Horse (87%) Elephant, Rabbit (80%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic 15 amino acid peptide from N-terminal extracellular domain of human PROKR2. Percent identity with other species by BLAST analysis: Human, Gibbon (100%), Monkey, Marmoset (93%), Horse (87%), Elephant, Rabbit (80%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (17 - 23 μg/mL)

Kommentare:

Target Species of Antibody: Human

Testdurchführung:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu Prokineticin Receptor 2

Target: Prokineticin Receptor 2 (PROKR2)

Andere Bezeichnung: PROKR2/Prokineticin Receptor 2 (PROKR2 Produkte)

Synonyme: GPR73L1 antikoerper, GPR73b antikoerper, GPRg2 antikoerper, HH3 antikoerper, KAL3 antikoerper, PKR2 antikoerper, dJ680N4.3 antikoerper, Gpr73l1 antikoerper, B830005M06Rik antikoerper, EG-VEGRF2 antikoerper, Gpcr73l1 antikoerper, prokineticin receptor 2 antikoerper, prokineticin receptor 1 antikoerper, PROKR2 antikoerper, Prokr2 antikoerper, PROKR1 antikoerper

Hintergrund: Name/Gene ID: PROKR2
Subfamily: Prokineticin
Family: GPCR

Synonyms: PROKR2, DJ680N4.3, GPCR73B, HH3, GPR73b, KAL3, GPRg2, Prokineticin receptor 2, PK-R2, PKR2, GPR73L1

Gen-ID: 128674

Pathways: Hedgehog Signalweg, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Myometrial Relaxation and Contraction, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma