RXFP2 Antikörper (Cytoplasmic Domain)

Produktnummer ABIN1049309

Produktdetails anti-RXFP2 Antikörper

Target: RXFP2 (Relaxin/insulin-Like Family Peptide Receptor 2 (RXFP2))

Bindungsspezifität: Cytoplasmic Domain

Reaktivität: Human, Maus, Pferd

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RXFP2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Spezifität: Human RXFP2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Kreuzreaktivität: Pferd

Homologie: Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Horse (100%) Monkey, Panda (93%) Dog, Pig, Xenopus (86%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic 14 amino acid peptide from 2nd cytoplasmic domain of human RXFP2. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Horse (100%), Monkey, Panda (93%), Dog, Pig, Xenopus (86%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 - 10 μg/mL), WB

Kommentare:

Target Species of Antibody: Human

Testdurchführung:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu RXFP2

Target: RXFP2 (Relaxin/insulin-Like Family Peptide Receptor 2 (RXFP2))

Andere Bezeichnung: RXFP2 / LGR8 (RXFP2 Produkte)

Synonyme: GPR106 antikoerper, GREAT antikoerper, INSL3R antikoerper, LGR8 antikoerper, LGR8.1 antikoerper, RXFPR2 antikoerper, Gpcr antikoerper, Lgr8 antikoerper, Gpr106 antikoerper, Great antikoerper, lgr8.1 antikoerper, rxfp2 antikoerper, rxfp2b antikoerper, zgc:63668 antikoerper, relaxin/insulin like family peptide receptor 2 antikoerper, relaxin/insulin-like family peptide receptor 2 antikoerper, relaxin/insulin-like family peptide receptor 2a antikoerper, RXFP2 antikoerper, Rxfp2 antikoerper, rxfp2a antikoerper

Hintergrund: Name/Gene ID: RXFP2
Subfamily: Relaxin
Family: GPCR

Synonyms: RXFP2, G-protein coupled receptor 106, GPR106, INSL3R, LGR8, GREAT, Relaxin receptor 2, LGR8.1, RXFPR2

Gen-ID: 122042

Pathways: cAMP Metabolic Process, Myometrial Relaxation and Contraction