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RXFP2 Antikörper (Cytoplasmic Domain)

RXFP2 Reaktivität: Human, Maus, Pferd WB, IHC, IHC (p) Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN1049309
  • Target Alle RXFP2 Antikörper anzeigen
    RXFP2 (Relaxin/insulin-Like Family Peptide Receptor 2 (RXFP2))
    Bindungsspezifität
    • 14
    • 11
    • 8
    • 5
    • 5
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cytoplasmic Domain
    Reaktivität
    • 49
    • 13
    • 8
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Human, Maus, Pferd
    Wirt
    • 51
    Kaninchen
    Klonalität
    • 51
    Polyklonal
    Konjugat
    • 26
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser RXFP2 Antikörper ist unkonjugiert
    Applikation
    • 28
    • 23
    • 6
    • 6
    • 5
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Spezifität
    Human RXFP2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
    Kreuzreaktivität
    Pferd
    Homologie
    Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Horse (100%) Monkey, Panda (93%) Dog, Pig, Xenopus (86%).
    Aufreinigung
    Immunoaffinity purified
    Immunogen
    Synthetic 14 amino acid peptide from 2nd cytoplasmic domain of human RXFP2. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Horse (100%), Monkey, Panda (93%), Dog, Pig, Xenopus (86%).

    Type of Immunogen: Synthetic peptide
    Top Product
    Discover our top product RXFP2 Primärantikörper
  • Applikationshinweise
    Approved: IHC, IHC-P (5 - 10 μg/mL), WB
    Kommentare

    Target Species of Antibody: Human

    Testdurchführung

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    Lot specific
    Buffer
    PBS, less than 0.1 % sodium azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    Haltbarkeit
    12 months
  • Target
    RXFP2 (Relaxin/insulin-Like Family Peptide Receptor 2 (RXFP2))
    Andere Bezeichnung
    RXFP2 / LGR8 (RXFP2 Produkte)
    Synonyme
    GPR106 antikoerper, GREAT antikoerper, INSL3R antikoerper, LGR8 antikoerper, LGR8.1 antikoerper, RXFPR2 antikoerper, Gpcr antikoerper, Lgr8 antikoerper, Gpr106 antikoerper, Great antikoerper, lgr8.1 antikoerper, rxfp2 antikoerper, rxfp2b antikoerper, zgc:63668 antikoerper, relaxin/insulin like family peptide receptor 2 antikoerper, relaxin/insulin-like family peptide receptor 2 antikoerper, relaxin/insulin-like family peptide receptor 2a antikoerper, RXFP2 antikoerper, Rxfp2 antikoerper, rxfp2a antikoerper
    Hintergrund
    Name/Gene ID: RXFP2
    Subfamily: Relaxin
    Family: GPCR

    Synonyms: RXFP2, G-protein coupled receptor 106, GPR106, INSL3R, LGR8, GREAT, Relaxin receptor 2, LGR8.1, RXFPR2
    Gen-ID
    122042
    Pathways
    cAMP Metabolic Process, Myometrial Relaxation and Contraction
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