SSTR1 Antikörper (C-Term)

Produktnummer ABIN1049356

Produktdetails anti-SSTR1 Antikörper

Target: SSTR1 (Somatostatin Receptor 1 (SSTR1))

Bindungsspezifität: C-Term

Reaktivität: Human, Maus, Ratte, Affe, Rind (Kuh), Hund, Kaninchen, Ziege, Pferd, Schaf, Schwein, Fledermaus, Hamster

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SSTR1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human SSTR1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Homologie: Percent identity with other species by BLAST analysis: Human, Monkey, Mouse, Rat, Sheep, Bovine, Bat, Dog, Goat, Hamster, Elephant, Panda, Horse, Rabbit, Pig (100%) Turkey, Chicken (87%) Zebrafish (80%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic 15 amino acid peptide from C-terminus of human SSTR1. Percent identity with other species by BLAST analysis: Human, Monkey, Mouse, Rat, Sheep, Bovine, Bat, Dog, Goat, Hamster, Elephant, Panda, Horse, Rabbit, Pig (100%), Turkey, Chicken (87%), Zebrafish (80%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (10 μg/mL)

Kommentare:

Target Species of Antibody: Human

Testdurchführung:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu SSTR1

Target: SSTR1 (Somatostatin Receptor 1 (SSTR1))

Andere Bezeichnung: SSTR1 (SSTR1 Produkte)

Synonyme: SSTR1 antikoerper, LOC100313735 antikoerper, SRIF-2 antikoerper, SS-1-R antikoerper, SS1-R antikoerper, SS1R antikoerper, Smstr-1 antikoerper, Smstr1 antikoerper, sst1 antikoerper, Gpcrrna antikoerper, somatostatin receptor 1 antikoerper, uncharacterized SSTR1 antikoerper, SSTR1 antikoerper, LOC100313735 antikoerper, Sstr1 antikoerper

Hintergrund: Name/Gene ID: SSTR1
Subfamily: Somatostatin
Family: GPCR

Synonyms: SSTR1, Somatostatin receptor type 1, SS1-R, SS1R, Somatostatin receptor 1, SRIF-2, Sst1 receptor, SS-1-R, Sst1

Gen-ID: 6751