TM9SF3 Antikörper (Internal Region)

Produktnummer ABIN1049414

Produktdetails anti-TM9SF3 Antikörper

Target: TM9SF3 (Transmembrane 9 Superfamily Member 3 (TM9SF3))

Bindungsspezifität: Internal Region

Reaktivität: Human, Maus, Rind (Kuh), Pferd, Ratte, Kaninchen, Hund, Meerschweinchen, Huhn, Schwein, Affe, Xenopus laevis

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TM9SF3 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human TM9SF3. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Homologie: Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Bovine, Dog, Horse, Rabbit, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Xenopus (100%) Lizard, Stickleback, Medaka (93%) Bat, Salmon, Zebrafish, Eye worm, Tick (87%) Pufferfish, Drosophila, Water flea, Nematode (80%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic 15 amino acid peptide from internal region of human TM9SF3. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Bovine, Dog, Horse, Rabbit, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Xenopus (100%), Lizard, Stickleback, Medaka (93%), Bat, Salmon, Zebrafish, Eye worm, Tick (87%), Pufferfish, Drosophila, Water flea, Nematode (80%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (10 μg/mL)

Kommentare:

Target Species of Antibody: Human

Testdurchführung:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu TM9SF3

Target: TM9SF3 (Transmembrane 9 Superfamily Member 3 (TM9SF3))

Andere Bezeichnung: TM9SF3 (TM9SF3 Produkte)

Synonyme: fb75h12 antikoerper, zgc:56246 antikoerper, wu:fa94f05 antikoerper, wu:fb75h12 antikoerper, SMBP antikoerper, EP70-P-iso antikoerper, 1810073M23Rik antikoerper, 2810031D16Rik antikoerper, AI115521 antikoerper, AI413748 antikoerper, AW146116 antikoerper, AW549777 antikoerper, Smbp antikoerper, mKIAA4036 antikoerper, RGD1564625 antikoerper, transmembrane 9 superfamily member 3 antikoerper, Transmembrane 9 superfamily member 3 antikoerper, TM9SF3 antikoerper, tm9sf3 antikoerper, LOC551549 antikoerper, Tsp_02755 antikoerper, tm9s3 antikoerper, Tm9sf3 antikoerper

Hintergrund: Name/Gene ID: TM9SF3

Synonyms: TM9SF3, EP70-P-iso, RP11-34E5.1, SMBP, SM-11044 binding protein, SM-11044-binding protein

Gen-ID: 56889