GPR175 Antikörper (Cytoplasmic Domain)

Produktnummer ABIN1049424

Produktdetails anti-GPR175 Antikörper

Target: GPR175 (TPRA1) (Transmembrane Protein, Adipocyte Asscociated 1 (TPRA1))

Bindungsspezifität: Cytoplasmic Domain

Reaktivität: Human, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GPR175 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Marke: IHC-plus™

Spezifität: Human TPRA1 / GPR175. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Homologie: Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Opossum (100%) Elephant, Turkey, Chicken, Xenopus, Seabass, Salmon, Pufferfish, Zebrafish, Stickleback (94%) Mouse, Rat, Bat, Dog, Bovine, Panda, Rabbit, Pig, Platypus, Lizard (88%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic 16 amino acid peptide from 1st cytoplasmic domain of human TPRA1 / GPR175. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Opossum (100%), Elephant, Turkey, Chicken, Xenopus, Seabass, Salmon, Pufferfish, Zebrafish, Stickleback (94%), Mouse, Rat, Bat, Dog, Bovine, Panda, Rabbit, Pig, Platypus, Lizard (88%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: ICC, IHC, IHC-P (8 - 19 μg/mL)

Kommentare:

Target Species of Antibody: Human

Testdurchführung:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu GPR175

Target: GPR175 (TPRA1) (Transmembrane Protein, Adipocyte Asscociated 1 (TPRA1))

Andere Bezeichnung: TPRA1 / GPR175 (TPRA1 Produkte)

Synonyme: GPR175 antikoerper, TMEM227 antikoerper, TPRA40 antikoerper, 40kDa antikoerper, Gpr175 antikoerper, Tpra40 antikoerper, transmembrane protein, adipocyte asscociated 1 antikoerper, transmembrane protein adipocyte associated 1 antikoerper, tpra1 antikoerper, TPRA1 antikoerper, Tpra1 antikoerper

Hintergrund: Name/Gene ID: TPRA1
Subfamily: Orphan-U
Family: GPCR

Synonyms: TPRA1, G protein-coupled receptor 175, Transmembrane protein 227, TMEM227, TPRA40, GPR175

Gen-ID: 131601