UTS2R Antikörper (N-Term)

Produktnummer ABIN1049452

Produktdetails anti-UTS2R Antikörper

Target: UTS2R (Urotensin 2 Receptor (UTS2R))

Bindungsspezifität: N-Term

Reaktivität: Human, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser UTS2R Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)

Marke: IHC-plus™

Spezifität: Human UTS2R / GPR14. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Homologie: Percent identity with other species by BLAST analysis: Human, Gibbon, Monkey (100%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic 20 amino acid peptide from N-terminal extracellular domain of human UTS2R / GPR14. Percent identity with other species by BLAST analysis: Human, Gibbon, Monkey (100%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (6 - 11 μg/mL)

Kommentare:

Target Species of Antibody: Human

Testdurchführung:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu UTS2R

Target: UTS2R (Urotensin 2 Receptor (UTS2R))

Andere Bezeichnung: UTS2R / GPR14 (UTS2R Produkte)

Synonyme: MGC84372 antikoerper, GPR14 antikoerper, UR-2-R antikoerper, UTR antikoerper, UTR2 antikoerper, Gpr14 antikoerper, Senr antikoerper, SENR antikoerper, urotensin 2 receptor antikoerper, urotensin 2 receptor L homeolog antikoerper, UTS2R antikoerper, uts2r.L antikoerper, Uts2r antikoerper

Hintergrund: Name/Gene ID: UTS2R
Subfamily: Urotensin
Family: GPCR

Synonyms: UTS2R, G protein-coupled receptor 14, GPR14, G-protein coupled receptor 14, UII-receptor, Urotensin 2 receptor, UT-II, UTR, UTR2, Uii-r, UR-2-R, Senr, Uii-r1a, Uiir, UR-II-R, Urotensin II receptor, Urotensin-2 receptor

Gen-ID: 2837