Ovalbumin Antikörper

Produktnummer ABIN105182

Hersteller Produkt- Nr.: 200-401-033

Produktdetails anti-Ovalbumin Antikörper

Target: Ovalbumin (OVA)

Reaktivität: Huhn

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Ovalbumin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC)

Hersteller Produkt- Nr.: 200-401-033

Hersteller: Rockland

Verwendungszweck: Ovalbumin Antibody

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Ovalbumin [Hen Egg White].

Produktmerkmale: Synonyms: rabbit anti-Ovalbumin Antibody, Egg albumin antibody, Plakalbumin, Allergen Gal d II, Allergen=Gal d 2, Hen Egg White

Aufreinigung: Rabbit Anti-Ovalbumin is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Ovalbumin [Hen Egg White]

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:300 - 1:2,000

Application Note: Anti-Ovalbumin Antibody has been tested in Western Blot. Although not tested this product could be useful in assays such as ELISA, IHC and IP. Specific conditions for reactivity and signal detection should be optimized by the end user.

Western Blot Dilution: 1:500 - 1:2,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen für anti-Ovalbumin Antikörper (ABIN105182)

Apperson, Vorachek, Dolan, Bobe, Pirelli, Hall: "Effects of feeding pregnant beef cows selenium-enriched alfalfa hay on passive transfer of ovalbumin in their newborn calves." in: Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS), Vol. 50, pp. 640-645, (2019) (PubMed).

Details zu Ovalbumin

Target: Ovalbumin (OVA)

Andere Bezeichnung: SERPINB14 (OVA Produkte)

Hintergrund: Background: Anti-Ovalbumin Antibody recognizes ovalbumin that is the main protein found in egg white. Ovalbumin makes up 55-65 % of the total protein. Ovalbumin displays sequence and three-dimensional homology to the serpin superfamily, but unlike most serpins, it does not undergo a conformational change upon proteolytic cleavage and is not a serine protease inhibitor. The function of ovalbumin is still unknown, although it is presumed to be a storage protein for tissue-specific, steroid hormone-induced gene expression. Ovalbumin is a key protein for biochemical investigations and processes and can be used as a control, blocking reagent, or antibody in assays such as ELISA, SDS-PAGE, IHC, Western Blot, and size exclusion chromatography.

Gen-ID: 396058

UniProt: P01012