Ovalbumin Antikörper (HRP)

Produktnummer ABIN105184

Hersteller Produkt- Nr.: 200-4333-0100

Produktdetails anti-Ovalbumin Antikörper (HRP)

Target: Ovalbumin (OVA)

Reaktivität: Huhn

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Ovalbumin Antikörper ist konjugiert mit HRP

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC)

Hersteller Produkt- Nr.: 200-4333-0100

Hersteller: Rockland

Verwendungszweck: Ovalbumin Antibody Peroxidase Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Ovalbumin [Hen Egg White].

Produktmerkmale: Synonyms: rabbit anti-Ovalbumin Antibody Peroxidase Conjugation, HRP conjugated rabbit anti-Ovalbumin antibody, Egg albumin antibody, Plakalbumin, Allergen Gal d II, Allergen=Gal d 2, Hen Egg White

Aufreinigung: Ovalbumin Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Anti-Ovalbumin Antibody was produced by repeated immunizations with hen egg white Ovalbumin protein.

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:500 - 1:2,500

Application Note: Anti-Ovalbumin Antibody Peroxidase Conjugated has been tested by ELISA and western blot and is suitable for IHC and IP. Researchers should determine optimal titers for applications that are not stated below.

Western Blot Dilution: 1:1,000 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:25,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Konservierungsmittel: Gentamicin sulfate

Vorsichtsmaßnahmen: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Referenzen für anti-Ovalbumin Antikörper (HRP) (ABIN105184)

Apperson, Vorachek, Dolan, Bobe, Pirelli, Hall: "Effects of feeding pregnant beef cows selenium-enriched alfalfa hay on passive transfer of ovalbumin in their newborn calves." in: Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS), Vol. 50, pp. 640-645, (2019) (PubMed).

Details zu Ovalbumin

Target: Ovalbumin (OVA)

Andere Bezeichnung: SERPINB14 (OVA Produkte)

Hintergrund: Background: Anti-Ovalbumin Antibody detects Ovalbumin. Ovalbumin is the main protein found in egg white, making up 60-65 % of the total protein. It is a non-inhibitory serpin. It is the storage protein of egg white. Anti-Ovalbumin Antibody is ideal for investigators involved in Cell Signaling, Immunology and Signal Transduction research.

Gen-ID: 396058

UniProt: P01012