Penicillinase Antikörper

Produktnummer ABIN105438

Produktdetails anti-Penicillinase Antikörper

Target: Penicillinase

Reaktivität: Enterobacter cloacae

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Penicillinase Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), ELISA

Kreuzreaktivität (Details): Cross reactivity against Penicillinase from other tissues and species may occur but have not been specifically determined.

Reinheit: Anti-PENICILLINASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Penicillinase [E.cloacae].

Endotoxin-Niveau: Low Endotoxin : No

Immunogen: Penicillinase [E.cloacae]
Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: PENICILLINASE ANTIBODY has been assayed against 1.0 µg of Penicillinase [E.cloacae] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:7.000 to 1:21.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:20.000
IF Immunoprecipitation Dilution: 1:100
Western Blot Dilution: 1:500 - 1:5.000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Handhabung: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Haltbarkeit: Expiration date is one (1) year from date of opening.

Details zu Penicillinase

Target: Penicillinase

Abstract: Penicillinase Produkte

Synonyme: beta-lactamase TEM-1 antikoerper, blaTEM-1 antikoerper

Hintergrund: Synonyms: Aer1 antibody, Beta lactamase 1 antibody, Beta lactamase AER 1 antibody, OXA9 antibody, Oxacillinase antibody, PenPC antibody

Gen-ID: 9125732

UniProt: P05364