Peroxidase Antikörper

Produktnummer ABIN105506

Produktdetails anti-Peroxidase Antikörper

Target: Peroxidase

Reaktivität: Meerrettich

Wirt: Meerschweinchen

Klonalität: Polyklonal

Konjugat: Dieser Peroxidase Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Kreuzreaktivität (Details): Cross reactivity against Peroxidase from other tissues and species may occur but have not been specifically determined.

Reinheit: Anti-Peroxidase (Horseradish) (Guinea Pig) Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Guinea Pig Serum as well as purified and partially purified Peroxidase [Horseradish].

Endotoxin-Niveau: Low Endotoxin : No

Immunogen: Peroxidase [Horseradish]
Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Anti-Peroxidase (Horseradish) (Guinea Pig) Antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity.
ELISA Dilution: 1:20.000 - 1:100.000
Immunohistochemistry Dilution: 1:1.000 - 1:5.000
Western Blot Dilution: 1:2.000 - 1:10.000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Handhabung: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Haltbarkeit: Expiration date is one (1) year from date of opening.

Details zu Peroxidase

Target: Peroxidase

Abstract: Peroxidase Produkte

Synonyme: peroxidase antikoerper, PER1 antikoerper, ShK domain and PerOxidase domain containing protein antikoerper, Peroxidase skpo-1 antikoerper, hypothetical protein antikoerper, peroxidase 1 antikoerper, 1-Cys-peroxiredoxine antikoerper, skpo-3 antikoerper, skpo-1 antikoerper, T06D8.10 antikoerper, C46A5.4 antikoerper, K10B4.1 antikoerper, R08F11.7 antikoerper, WSP1 antikoerper, PER1 antikoerper

Hintergrund: Horse Radish Peroxidase (HRP) is an enzyme that utilize organic peroxide compounds as electron donors. Naturally provides protection for plants against pathogens, but can be utilized in molecular biology to convert various substrates to detectable compounds (such as in Western Blotting and ELISAs). Anti-Peroxidase (Horseradish) (Guinea Pig) Antibody is ideal for investigators in Microbiology, Immunology, and Cell Biology research. Synonyms: HRP, Horseradish peroxidase, Guinea pig-anti-peroxidase antibody