PLG Antikörper

Produktnummer ABIN105702

Hersteller Produkt- Nr.: 200-101-208-0100

Produktdetails anti-PLG Antikörper

Target: PLG (Plasminogen (PLG))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser PLG Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Hersteller Produkt- Nr.: 200-101-208-0100

Hersteller: Rockland

Verwendungszweck: Plasminogen Antibody

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Plasminogen [Human Plasma].

Produktmerkmale: Synonyms: goat anti-Plasminogen Antibody, Angiostatin antibody, Microplasmin antibody, Plasmin antibody, Plasmin heavy chain A antibody, Plasmin light chain B antibody, PLG antibody

Aufreinigung: Anti-Plasminogen is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Plasminogen [Human Plasma]

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti-Plasminogen Antibody has been tested by western blot. This product is suitable to be assayed against 1.0 μg of Plasminogen [Human Plasma] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) code #605-4302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:16,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:2,000 - 1:10,000

ELISA Dilution: 1:20,000 - 1:100,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 10.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu PLG

Target: PLG (Plasminogen (PLG))

Andere Bezeichnung: PLG (PLG Produkte)

Hintergrund: Background: Plasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. In ovulation, it weakens the walls of the Graafian follicle. It activates the urokinase-type plasminogen activator, collagenases, and several complement zymogens, such as C1 and C5. The cleavage of fibronectin and laminin leads to cell detachment and apoptosis. Also, it cleaves fibrin, thrombospondin, and von Willebrand factor. Its has roles in tissue remodeling and tumor invasion which may be modulated by CSPG4 and binds to cells. Angiostatin is an angiogenesis inhibitor that blocks neovascularization and growth of experimental primary and metastatic tumors in vivo. Plasmin is inactivated by alpha-2-antiplasmin immediately after dissociation from the clot.

Gen-ID: 5340

UniProt: P00747

Pathways: Komplementsystem, Lipid Metabolism