PPARA Antikörper (N-Term)
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- Target Alle PPARA Antikörper anzeigen
- PPARA (Peroxisome Proliferator-Activated Receptor alpha (PPARA))
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Bindungsspezifität
- N-Term
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Reaktivität
- Human, Maus
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser PPARA Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Fluorescence Microscopy (FM)
- Hersteller Produkt- Nr.
- 600-401-421
- Hersteller
- Rockland
- Verwendungszweck
- PPAR alpha Antibody
- Kreuzreaktivität (Details)
- Anti-PPAR alpha Antibody is directed against mouse PPAR alpha protein.
- Produktmerkmale
- Synonyms: rabbit anti-Ppar alpha antibody, Pparα, Peroxisome proliferator-activated receptor alpha, PPAR-alpha, Nuclear receptor subfamily 1 group C member 1, Ppar-a, Nr1c1, Ppar
- Aufreinigung
- The product was affinity purified from monospecific antiserum by immunoaffinity purification.
- Sterilität
- Sterile filtered
- Immunogen
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Immunogen: PPAR alpha Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a N-Terminal region near amino acids 1-25 of mouse PPAR alpha.
Immunogen Type: Conjugated Peptide
- Isotyp
- IgG
- Top Product
- Discover our top product PPARA Primärantikörper
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- Applikationshinweise
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Flow Cytometry Dilution: User Optimized
Immunohistochemistry Dilution: 1:100-1:300
Application Note: Anti-PPAR alpha Antibody has been tested in ELISA, Western Blot, Immunohistochemistry, and Immunofluorescence. Expect a single band approximately 52 kDa in size corresponding to PPAR alpha by western blot in the appropriate tissue or cell lysate. A 1:200 dilution is suggested for Immunohistochemistry. Specific conditions for reactivity should be optimized by the end user.
Western Blot Dilution: 1:500 - 1:2,000
ELISA Dilution: 1:75,000 - 1:125,000
IF Microscopy Dilution: 1-5 μg/mL
Other: User Optimized
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 1.0 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Haltbarkeit
- 12 months
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Fenofibrate induces effective apoptosis in mantle cell lymphoma by inhibiting the TNFalpha/NF-kappaB signaling axis." in: Leukemia, Vol. 24, Issue 8, pp. 1476-86, (2010) (PubMed).
: "Medicine. PPARs as therapeutic targets: reverse cardiology?" in: Science (New York, N.Y.), Vol. 302, Issue 5644, pp. 406-7, (2003) (PubMed).
: "Human and rat peroxisome proliferator activated receptors (PPARs) demonstrate similar tissue distribution but different responsiveness to PPAR activators." in: The Journal of steroid biochemistry and molecular biology, Vol. 51, Issue 3-4, pp. 157-66, (1995) (PubMed).
: "
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Fenofibrate induces effective apoptosis in mantle cell lymphoma by inhibiting the TNFalpha/NF-kappaB signaling axis." in: Leukemia, Vol. 24, Issue 8, pp. 1476-86, (2010) (PubMed).
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- Target
- PPARA (Peroxisome Proliferator-Activated Receptor alpha (PPARA))
- Andere Bezeichnung
- Ppara (PPARA Produkte)
- Hintergrund
- Background: Since their discovery in the early 1990's, the peroxisome proliferator activated receptors (PPARs) have attracted significant attention. This is primarily because PPARs serve as receptors for two very important classes of drugs: the hypolipidemic fibrates and the insulin sensitizing thiazolidinediones. Peroxisome proliferators are non-genotoxic carcinogens that are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed PPARs. Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Upon binding fatty acids or hypolipidemic drugs, PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate the expression of target genes. There are 3 known subtypes of PPARs: PPAR-alpha, PPAR-delta and PPAR-gamma. Mostly target genes are involved in the catabolism of fatty acids. Conversely, PPAR-gamma is activated by peroxisome proliferators such as prostaglandins, leukotrienes and Anti diabetic thiazolidinediones and affects the expression of genes involved in the storage of the fatty acids. PPAR-gamma may also be involved in adipocyte differentiation. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase and cytochrome P450 through interaction with specific response elements. Anti-Ppar Antibody is useful for research interested in transcription and metabolic pathways.
- Gen-ID
- 19013, 31543500
- UniProt
- P23204
- Pathways
- Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha, Regulation of Carbohydrate Metabolic Process, Hepatitis C
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