Protein G Antikörper
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- Target Alle Protein G Antikörper anzeigen
- Protein G
- Reaktivität
- Streptococcus
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser Protein G Antikörper ist unkonjugiert
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Applikation
- ELISA, Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Fluorescence Microscopy (FM)
- Hersteller Produkt- Nr.
- 200-401-138-0100
- Hersteller
- Rockland
- Verwendungszweck
- Protein G Antibody
- Kreuzreaktivität (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Protein G.
- Produktmerkmale
- Synonyms: rabbit anti-Protein G Antibody, Rabbit anti Protein G, Rabbit anti PG
- Aufreinigung
- Anti-Protein G is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
- Immunogen
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Immunogen: Protein G [Streptococcus species]
Immunogen Type: Native Protein
- Isotyp
- IgG
- Top Product
- Discover our top product Protein G Primärantikörper
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- Applikationshinweise
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Flow Cytometry Dilution: User Optimized
Immunohistochemistry Dilution: User Optimized
Application Note: Anti-Protein G is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Western Blot Dilution: 1:2,000 - 1:10,000
ELISA Dilution: 1:20,000 - 1:100,000
IF Microscopy Dilution: User Optimized
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Haltbarkeit
- 12 months
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- Target
- Protein G
- Abstract
- Protein G Produkte
- Hintergrund
- Background: Protein G is a protein that has the property of binging to immunoglobulins. It is a 65- kDa cell surface protein that is commonly used for purifying antibodies through binding to the Fab and Fc regions. Protein G was originally isolated from Streptococcal bacteria. It is similar in properties to Protein A but has unique IgG binding specificities. Native protein G also binds albumin, however Rockland uses recombinant forms of Protein G that only bind to immunoglobulins.
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