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Protein G Antikörper

Rockland 200-401-138-0100 Reaktivität: Streptococcus ELISA, FACS, WB, IHC, FM Wirt: Kaninchen Polyclonal unconjugated
Rockland
Produktnummer ABIN106214
Hersteller Produkt- Nr.: 200-401-138-0100
  • Target Alle Protein G Antikörper anzeigen
    Protein G
    Reaktivität
    Streptococcus
    Wirt
    • 8
    • 1
    Kaninchen
    Klonalität
    • 9
    Polyklonal
    Konjugat
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Dieser Protein G Antikörper ist unkonjugiert
    Applikation
    • 6
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Fluorescence Microscopy (FM)
    Hersteller Produkt- Nr.
    200-401-138-0100
    Hersteller
    Rockland
    Verwendungszweck
    Protein G Antibody
    Kreuzreaktivität (Details)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Protein G.
    Produktmerkmale
    Synonyms: rabbit anti-Protein G Antibody, Rabbit anti Protein G, Rabbit anti PG
    Aufreinigung
    Anti-Protein G is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
    Immunogen

    Immunogen: Protein G [Streptococcus species]

    Immunogen Type: Native Protein

    Isotyp
    IgG
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    Discover our top product Protein G Primärantikörper
  • Applikationshinweise

    Flow Cytometry Dilution: User Optimized

    Immunohistochemistry Dilution: User Optimized

    Application Note: Anti-Protein G is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.

    Western Blot Dilution: 1:2,000 - 1:10,000

    ELISA Dilution: 1:20,000 - 1:100,000

    IF Microscopy Dilution: User Optimized

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
    Haltbarkeit
    12 months
  • Target
    Protein G
    Abstract
    Protein G Produkte
    Hintergrund
    Background: Protein G is a protein that has the property of binging to immunoglobulins. It is a 65- kDa cell surface protein that is commonly used for purifying antibodies through binding to the Fab and Fc regions. Protein G was originally isolated from Streptococcal bacteria. It is similar in properties to Protein A but has unique IgG binding specificities. Native protein G also binds albumin, however Rockland uses recombinant forms of Protein G that only bind to immunoglobulins.
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