Pyruvate Kinase Antikörper

Produktnummer ABIN106387

Produktdetails anti-Pyruvate Kinase Antikörper

Target: Pyruvate Kinase (PK)

Reaktivität: Kaninchen

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Pyruvate Kinase Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Conjugation (Con), Dot Blot (DB)

Produktmerkmale: Concentration Definition: by Refractometry

Aufreinigung: This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Pyruvate Kinase [Rabbit Muscle].

Immunogen: Pyruvate Kinase [Rabbit Muscle]
Immunogentype:Native

Anwendungsinformationen

Applikationshinweise: Anti-PYRUVATE KINASE Antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity. Specific conditions should be optimized by user.

Kommentare:

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Restore with deionized water (or equivalent)

Konzentration: 40 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Referenzen für anti-Pyruvate Kinase Antikörper (ABIN106387)

Shimada, Shinagawa, Ishii: "Modulation of M2-type pyruvate kinase activity by the cytoplasmic PML tumor suppressor protein." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 13, Issue 3, pp. 245-54, (2008) (PubMed).

Details zu Pyruvate Kinase

Target: Pyruvate Kinase (PK)

Andere Bezeichnung: Pyruvate Kinase (PK Produkte)

Synonyme: CG7070 antikoerper, Dmel\\CG7070 antikoerper, PYK antikoerper, Pyk antikoerper, PYK3b antikoerper, PYK3c antikoerper, Pyruvate kinase antikoerper, PK antikoerper, pyruvate kinase antikoerper, pyruvate kinase (PK) antikoerper, Pyruvate kinase (PK) antikoerper, pyruvate kinase PKM antikoerper, testis expressed 45 antikoerper, PyK antikoerper, pykA antikoerper, PYK3a|PYK3b|PYK3c antikoerper, pyk antikoerper, pyk1 antikoerper, pk antikoerper, LOC100534543 antikoerper, TEX45 antikoerper

Hintergrund: Pyruvate kinase (EC: 2.7.1.40) is an enzyme involved in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule of pyruvate and one molecule of ATP. This process also requires a magnesium ion. The enzyme is a 'transferase' under the international classification of enzymes. This step is the final one in the glycolytic pathway, which produces pyruvate molecules, the final product of aerobic glycolysis. However, in anaerobic glycolysis, lactate dehydrogenase will utilize the NADH produced by glyceraldehyde phosphate dehydrogenase to reduce pyruvate to lactate. In humans, there are two pyruvate kinase isozymes: type M (muscle, SwissProt P14618) and type L,R (liver and erythrocyte, SwissProt P30613). The isozymes differ in primary structure and regulation. This reaction has a large positive free energy change, one of three in glycolysis. All three such steps regulate the overall activity of the pathway, and are, in general, irreversible under wild-type conditions. Anti-Pyruvate kinase antibody is idea for investigators involved in cytokines and growth factor research.
Synonyms: Pyruvate kinase isozymes M1/M2 antibody, Pyruvate kinase muscle isozyme antibody, Pyruvate kinase 2/3 antibody, Cytosolic thyroid hormone-binding protein antibody

Gen-ID: 100008676

NCBI Accession: NP_001182574

UniProt: P11974