RFX5 Antikörper

Produktnummer ABIN106544

Hersteller Produkt- Nr.: 200-401-194

Produktdetails anti-RFX5 Antikörper

Target: RFX5 (Regulatory Factor X 5 (RFX5))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RFX5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Gel Shift (GS), Chromatin Immunoprecipitation (ChIP)

Hersteller Produkt- Nr.: 200-401-194

Hersteller: Rockland

Verwendungszweck: RFX5 Antibody

Kreuzreaktivität (Details): Anti-RFX5 (aa 320 to 494) may react with unknown minor bands at 65 kDa and 80 kDa.

Produktmerkmale: Synonyms: rabbit anti-RFX5 antibody, DNA binding protein RFX 5 antibody, Influences HLA class II expression antibody, Regulatory factor X 5 antibody, Regulatory factor X subunit 5 antibody, Regulatory factor X 5 (influences HLA class II expression) antibody

Aufreinigung: RFXF5 antibody was prepared from monospecific antiserum by delipidation, salt fractionation and ion exchange chromatography.

Sterilität: Sterile filtered

Immunogen:

Immunogen: RFX5 peptide corresponding to a region at amino acids 320 to 494 of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

Immunogen Type: Conjugated Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Gel Shift Dilution: 0.5 μL to 1.0 μL

Application Note: Anti RFX5 antibody has been assayed by immunoblot and found to be reactive against RFX5 (aa 320 to 494) from a variety of fibroblast and B-cell lysates at a dilution of 1:1,000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG.  Anti-RFX5 (aa 320 to 494) detects a 75 kDa band by immunoblot for human RFX5.  This product was also tested in a gel supershift assay and found to be reactive against RFX5 complexes using 0.5 to 1.0 μL per assay.

ChIP Dilution: User Optimized

Western Blot Dilution: 1:1,000

ELISA Dilution: 1:5,000 - 1:25,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen für anti-RFX5 Antikörper (ABIN106544)

Zhao, Xie, Liao, Zhang, Cao, Fei, Wang, Wei, Shao, Chen: "The transcription factor RFX5 is a transcriptional activator of the TPP1 gene in hepatocellular carcinoma." in: Oncology reports, Vol. 37, Issue 1, pp. 289-296, (2017) (PubMed).

Dille, Kleinschnitz, Kontchou, Nölke, Häcker: "In contrast to Chlamydia trachomatis, Waddlia chondrophila grows in human cells without inhibiting apoptosis, fragmenting the Golgi apparatus, or diverting post-Golgi sphingomyelin transport." in: Infection and immunity, Vol. 83, Issue 8, pp. 3268-80, (2015) (PubMed).

Xu, Sengupta, Seto, Smith: "Regulatory factor for X-box family proteins differentially interact with histone deacetylases to repress collagen alpha2(I) gene (COL1A2) expression." in: The Journal of biological chemistry, Vol. 281, Issue 14, pp. 9260-70, (2006) (PubMed).

Details zu RFX5

Target: RFX5 (Regulatory Factor X 5 (RFX5))

Andere Bezeichnung: RFX5 (RFX5 Produkte)

Hintergrund: Background: Anti-RFX5 is specific for the RFX5 protein. RFX5 is the fifth member of the growing family of DNA-binding proteins sharing a novel and highly characteristic DNA-binding domain called the RFX motif. Multiple alternatively spliced transcript variants have been found but the full-length natures of only two have been determined. RFX5 has been shown to interact with CIITA.

Gen-ID: 5993

UniProt: P48382