Rhodamine Antikörper

Produktnummer ABIN106554

Hersteller Produkt- Nr.: 200-301-246

Produktdetails

Target: Rhodamine

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Dot Blot (DB)

Hersteller Produkt- Nr.: 200-301-246

Hersteller: Rockland

Verwendungszweck: Rhodamine Antibody

Kreuzreaktivität (Details): Rhodamine isomer 5 and isomer 6 are reactive as TAMRA, as well as TRITC conjugated proteins.

Produktmerkmale: Synonyms: Mouse Anti-Rhodamine Antibody

Aufreinigung: RHODAMINE Monoclonal Antibody was protein A purified and reacts specifically with Rhodamine and its derivatives.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Anti-RHODAMINE Monoclonal Antibody was produced after repeated immunizations of balb/c mice with rhodamine conjugated KLH.

Immunogen Type: Other

Klon: 11H10

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:1,000 - 1:10,000

Application Note: This protein A purified monoclonal antibody against rhodamine reacts with most derivative molecules and has been tested for use in ELISA. Optimal concentration in other immunoassays should be determined by the researcher.

Western Blot Dilution: 1:1,000 - 1:10,000

ELISA Dilution: 1:10,000 - 1:30,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen

Shimamura, Sasaki, Tanaka: "The Src substrate SKAP2 regulates actin assembly by interacting with WAVE2 and cortactin proteins." in: The Journal of biological chemistry, Vol. 288, Issue 2, pp. 1171-83, (2013) (PubMed).

Antigendetails

Target: Rhodamine

Substanzklasse: Chemical

Hintergrund: Background: RHODAMINE Monoclonal Antibody specifically detect dyes in the Rhodamine family. Rhodamine is a family member of the fluorone dyes. Examples are Rhodamine 6G and Rhodamine B. They are often used as a tracer dye within water to determine the rate and direction of flow and transport. Rhodamine dyes fluoresce and can thus be detected easily and inexpensively with instruments called fluorometers. Rhodamine dyes are used extensively in biotechnology applications such as fluorescence microscopy, flow cytometry, fluorescence correlation spectroscopy and ELISA.