Western Blotting (WB), Immunoprecipitation (IP), ELISA
Kreuzreaktivität (Details)
Cross reactivity against Ribonuclease A from other tissues and species may occur but have not been specifically determined.
Reinheit
Anti-RIBONUCLEASE A is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Ribonuclease A [Bovine pancreas].
Endotoxin-Niveau
Low Endotoxin : No
Immunogen
Ribonuclease A [Bovine pancreas] Immunogen Type: Native Protein
RIBONUCLEASE A Antibodyhas been assayed against 1.0 µg of Ribonuclease A [Bovine pancreas] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2’-azino-bis-[3-ethylbenthiozoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:40.000 to 1:180.000 is suggested for this product. ELISA Dilution: 1:5.000 - 1:20.000 IF Immunoprecipitation Dilution: 1:100 Western Blot Dilution: 1:500 - 1:5.000
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Handhabung
Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Lagerung
-20 °C
Informationen zur Lagerung
Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
Haltbarkeit
Expiration date is one (1) year from date of opening.
Target
RNASE1
(Ribonuclease, RNase A Family, 1 (Pancreatic) (RNASE1))
RNASE1 antikoerper, RNS1 antikoerper, ATRNS1 antikoerper, RIBONUCLEASE 1 antikoerper, T17M13.16 antikoerper, T17M13_16 antikoerper, ribonuclease 1 antikoerper, RIB1 antikoerper, AI574248 antikoerper, Rib-1 antikoerper, Rib1 antikoerper, SRN antikoerper, ribonuclease A family member 1, pancreatic antikoerper, ribonuclease pancreatic-like antikoerper, ribonuclease A family member k6 antikoerper, Ribonuclease 1 antikoerper, ribonuclease 1 antikoerper, ribonuclease, RNase A family, 1 (pancreatic) antikoerper, ribonuclease pancreatic antikoerper, RNASE1 antikoerper, LOC475395 antikoerper, RNASE6 antikoerper, Rnase1 antikoerper, RNS1 antikoerper, LOC101113761 antikoerper
Hintergrund
Synonyms: Pancreatic ribonuclease antibody, Rib 1 antibody, Rib1 antibody, Ribonuclease a family 1 antibody, Ribonuclease pancreatic antibody, Ribonuclease RNase A family 1 antibody, Ribonuclease RNase A family 1 pancreatic antibody