NEDD8 Antikörper

Produktnummer ABIN106594

Produktdetails anti-NEDD8 Antikörper

Target: NEDD8 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8))

Reaktivität: Saccharomyces cerevisiae

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NEDD8 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktmerkmale: Concentration Definition: by UV absorbance at 280 nm

Immunogen: Rub1 Antibody was prepared from rabbit serum after repeated immunizations with full-length recombinant yeast Rub1 protein.
Immunogentype:Recombinant

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Rub1 Antibody is deected as a 6 kDa band corresponding to yeast Rub1in immunoblotting.  Most yeast cell lysates can be used as a positive control without induction or stimulation.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Referenzen für anti-NEDD8 Antikörper (ABIN106594)

Toro, Toth, Petroski: "The cyclomodulin cycle inhibiting factor (CIF) alters cullin neddylation dynamics." in: The Journal of biological chemistry, Vol. 288, Issue 21, pp. 14716-26, (2013) (PubMed).

Details zu NEDD8

Target: NEDD8 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8))

Andere Bezeichnung: Rub1 (NEDD8 Produkte)

Synonyme: NEDD-8 antikoerper, Rub1 antikoerper, CG10679 antikoerper, Dmel\\CG10679 antikoerper, NEDD8 antikoerper, dNEDD8 antikoerper, nedd8 antikoerper, ubiquitin antikoerper, si:zc14a17.5 antikoerper, GB17785 antikoerper, DDBDRAFT_0218116 antikoerper, DDBDRAFT_0238041 antikoerper, DDB_0218116 antikoerper, DDB_0238041 antikoerper, nedd8.S antikoerper, neural precursor cell expressed, developmentally down-regulated 8 antikoerper, neural precursor cell expressed, developmentally down-regulated gene 8 antikoerper, CG10679 gene product from transcript CG10679-RB antikoerper, Nedd8 antikoerper, ubiquitin-like protein Nedd8 antikoerper, neural precursor cell expressed, developmentally down-regulated 8 L homeolog antikoerper, NEDD8 antikoerper, Nedd8 antikoerper, nedd8 antikoerper, nedd8.L antikoerper

Hintergrund: Ubiquitin-like proteins fall into  two classes: the first class, ubiquitin-like modifiers (UBL’s) function as modifiers in a manner analogous to that of ubiquitin.  Examples of UBL’s are SUMO, Rub1 (also called Nedd8), Apg12, and Hub1.  Proteins of the second class include parkin, RAD23, and DSK2, and are designated ubiquitin-domain proteins (UDP’s). These proteins contain domains that are related to ubiquitin but are otherwise unrelated to each other.  In contrast to UBL’s, UDP’s are not proteolytically processed or conjugated to other proteins.  Rub1, and the corresponding human homolog Nedd8, are activated by the E1 ubiquitin-activating enzyme UBA2, which forms isopeptide linkages between thioesters.  Nedd8 shows 80% homology to ubiquitin.  The best known targets of Rub1 modification are members of the cullin family.  Cullins are subunits of an E3-ubiquitin ligase complex called the Skp1/Cul1/Cdc53-F-box (SCF).  The SCF promotes transfer of ubiquitin from a ubiquitin conjugating enzyme (E2) to the target protein.  Rub1 modification may regulate SCF function or localization.
Synonyms: NEDD8-like protein RUB1, Ubiquitin-like protein RUB1

Gen-ID: 851717

UniProt: Q03919

Pathways: Ubiquitin Proteasome Pathway