PIPOX Antikörper

Produktnummer ABIN106699

Hersteller Produkt- Nr.: 200-101-093-0100

Produktdetails anti-PIPOX Antikörper

Target: PIPOX (Pipecolic Acid Oxidase (PIPOX))

Reaktivität: Microbial

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser PIPOX Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Immunoprecipitation (IP), Dot Blot (DB)

Hersteller Produkt- Nr.: 200-101-093-0100

Hersteller: Rockland

Verwendungszweck: Sarcosine Oxidase Antibody

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Sarcosine Oxidase [Microbial].

Produktmerkmale: Synonyms: goat anti-Sarcosine Oxidase Antibody, Sarcosine oxidase subunit alpha, Sarcosine oxidase subunit

Aufreinigung: Anti-Sarcosine Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Sarcosine Oxidase [Microbial]

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti-Sarcosine Oxidase Antibody has been tested by dot blot and western blot and is suitable for ELISA assay against 1.0 μg of Sarcosine Oxidase [Microbial] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) code #605-4302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:3,000 to 1:12,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 10.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Details zu PIPOX

Target: PIPOX (Pipecolic Acid Oxidase (PIPOX))

Andere Bezeichnung: Sarcosine Oxidase (PIPOX Produkte)

Hintergrund: Background: Sarcosine Oxidase catalyzes the oxidative demethylation of sarcosine to yield glycine, hydrogen peroxide and 5,10-methylenetetrahydrofolate.

Gen-ID: 10586229

NCBI Accession: YP_004446819

UniProt: Q46337