Streptavidin Antikörper (HRP)

Produktnummer ABIN107094

Produktdetails anti-Streptavidin Antikörper (HRP)

Target: Streptavidin

Reaktivität: Streptomyces avidinii

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Streptavidin Antikörper ist konjugiert mit HRP

Applikation: ELISA

Produktmerkmale: Concentration Definition: by UV absorbance at 280 nm

Immunogen: Streptavidin [Streptomyces avidinii]
Immunogentype:Native

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: This product has been assayed against 1.0 ug of Streptavidin in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) for 30 minutes at room temperature. Optimal concentrations in immunoassyas should be determined by researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Restore with deionized water (or equivalent)

Konzentration: 10.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Konservierungsmittel: Gentamicin sulfate

Handhabung: Do NOT add Sodium Azide!

Lagerung: 4 °C

Details zu Streptavidin

Target: Streptavidin

Abstract: Streptavidin Produkte

Hintergrund: Anti-streptavidin antibody detects Streptavidin, a 60 kDa protein purified from the bacterium Streptomyces avidinii. Streptavidin is a homo-tetrameric protein having a very high affinity for biotin (vitamin B7). Streptavidin bound biotin has a dissociation constant (Kd) on the order of about 10^−14 mol/L. Streptavidin and biotin are used extensively in molecular biology and bionanotechnology due to the resistance of streptavidin-biotin complex to organic solvents, denaturants (e.g. guanidinium chloride), detergents (e.g. SDS, Triton), proteolytic enzymes, and extremes of temperature and pH.
Synonyms: HRP