Concentration Definition: by UV absorbance at 280 nm
Immunogen
This antibody was purified from rabbit serum after repeated immunizations with recombinant human Thymidylate Synthase (36 kDa) produced in E.coli. Immunogentype:Recombinant
TYMS
Reaktivität: Human, Maus, Ratte
IF
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
THYMIDYLATE SYNTHASE antibody has been tested for use in ELISA, immunoprecipitation, immunofluorescence microscopy, immunohistochemistry and immunoblotting. The antibody recognizes the expected additional band corresponding to the ternary complex of hTS-dFUMP-reduced folate in HeLa cells treated with the TS inhibitor 5-FUdR. This event occurs in most human breast, colorectal, gastric, head and neck carcinomas. The antibody recognizes the 36,000 MW hTS. Reactivity in other immunoassays is unknown.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Restore with deionized water (or equivalent)
Konzentration
10.0 mg/mL
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.