Calmodulin,CAM Antikörper (AA 1-149)
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- Target Alle Calmodulin,CAM Antikörper anzeigen
- Calmodulin,CAM
- Bindungsspezifität
- AA 1-149
- Reaktivität
- Ratte
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser Calmodulin,CAM Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Immunocytochemistry (ICC), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Sequenz
- MGHHHHHHSG S- MADQLTEEQI AEFKEAFSLF DKDGDGTITT KELGTVMRSL GQNPTEAELQ DMINEVDADG NGTIDFPEFL TMMARKMKDT DSEEEIREAF RVFDKDGNGY ISAAELRHVM TNLGEKLTDE EVDEMIREAD IDGDGQVNYE EFVQMMTAK
- Spezifität
- It has been selected for its ability to recognize CAM in immunohistochemical staining and Western blotting.
- Aufreinigung
- Affinity Chromatography
- Immunogen
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Recombinant CAM expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against CAM. - Isotyp
- IgG
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- Applikationshinweise
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Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user. - Kommentare
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Content: The quality control contains recombinant CAM (Met1~Lys149) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % . - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- Lot specific
- Buffer
- Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handhabung
- Avoid repeated freeze/thaw cycles
- Lagerung
- 4 °C
- Informationen zur Lagerung
- Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
- Haltbarkeit
- 12 months
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- Target
- Calmodulin,CAM
- Andere Bezeichnung
- Calmodulin (CAM) (Calmodulin,CAM Produkte)
- Synonyme
- CALM2 antikoerper, CaMA antikoerper, R75142 antikoerper, PHKD antikoerper, PHKD3 antikoerper, calmodulin 1 antikoerper, calmodulin 2 antikoerper, calmodulin 3 antikoerper, CALM1 antikoerper, Calm2 antikoerper, CALM2 antikoerper, Calm3 antikoerper, CALM3 antikoerper
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