Reaktivität: Human
ELISA
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
Anti-Alpha-1-Anti-Trypsin has been assayed against 1.0 ug of a-1-anti-Trypsin in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:8,000 to 1:29,000 of the reconstitution concentration is suggested for this product.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Restore with deionized water (or equivalent)
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Konservierungsmittel
Gentamicin sulfate
Handhabung
Do NOT add Sodium Azide!
Lagerung
4 °C
Target
Alpha1 -Trypsin
Andere Bezeichnung
Alpha-1-Trypsin
Hintergrund
A member of the serpin family, Anti-Alpha-1-Anti-Trypsin inhibits serine proteases. With a moderate affinity for plasmin and thrombin, Anti-Alpha-1-Anti-Trypsin antibody mainly targets elastase. It inhibits trypsin, chymotrypsin and plasminogen activator by inhibiting insulin-induced NO synthesis. It also decreases coagulation time and has proteolytic activity against insulin and plasmin. The short peptide from AAT also targets elastase, but not trypsin. Anti-Alpha-1-Anti-Trypsin is ideal for researchers involved in Metabolism, Cancer, and Cardiovascular research. Synonyms: A1AT antibody, AAT antibody, Alpha 1 antiproteinase antibody, Alpha 1 antitrypsin antibody, Alpha 1 protease inhibitor antibod