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p53 Antikörper (AA 108-370)

Name: p53 Antikörper (AA 108-370)
SKU: ABIN1078633
Price: 265.2 EUR
Availability: InStock

TP53 Reaktivität: Human WB, IHC, IP, ICC Wirt: Kaninchen Polyclonal unconjugated

Western Blotting (WB) image for anti-Tumor Protein P53 (TP53) (AA 108-370) antibody (ABIN1078633) (p53 Antikörper (AA 108-370))

Used in DAB staining on fromalin fixed paraffin-embedded Kidney tissue (p53 Antikörper (AA 108-370))

IHC

Knockout Varification: Lane 1: Wild-type MCF7 cell lysate; Lane 2: TP53 knockout MCF7 cell lysate; Predicted MW: 24,30,33,34,38,39,44kDa Observed MW: 46kDa Primary Ab: 1µg/ml Rabbit Anti-Human TP53 Antibody Second Ab: 0. (p53 Antikörper (AA 108-370))

4 Abbildungen

(1 reference)

Produktnummer ABIN1078633

Datenblatt (PDF)

Target Alle p53 (TP53) Antikörper anzeigen

p53 (TP53) (Tumor Protein P53 (TP53))
Bindungsspezifität
43

40

37

30

29

29

26

26

24

22

20

20

19

19

18

18

16

15

13

9

8

8

8

7

7

6

6

6

6

6

6

6

5

5

4

4

4

4

4

4

4

4

4

4

3

3

3

3

3

3
AA 108-370
Reaktivität
835

255

211

107

50

34

33

19

10

9

7

6

6

3

3

3

3

2

2

2

2

1

1

1
Human
Wirt
623

229

6

2

1
Kaninchen
Passende Sekundärantikörper
Klonalität
559

301

1
Polyklonal
Konjugat
485

38

32

19

19

13

13

13

13

13

13

13

13

13

12

12

11

11

11

11

11

11

11

5

5

5

5

5

5

2

2

2

2

2

2

2

1

1

1

1

1

1
Dieser p53 Antikörper ist unkonjugiert
Applikation
668

298

250

202

200

149

128

124

119

68

59

35

18

16

13

12

9

9

8

6

4

3

3

3

3

2

2

2

1

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Verwendungszweck

Polyclonal Antibody to Tumor Protein p53 (P53)

Spezifität

The antibody is a rabbit polyclonal antibody raised against P53. It has been selected for its ability to recognize P53 in immunohistochemical staining and western blotting.

Kreuzreaktivität

Ratte

Aufreinigung

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen

Recombinant Tumor Protein p53 (P53) corresdonding to Gly108~Lys370 with N-terminal His Tag

Isotyp

IgG

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Applikationshinweise

Western blotting: 0.3-2 μg/mL
Immunohistochemistry: 5-20 μg/mL
Immunocytochemistry: 5-20 μg/mL
Optimal working dilutions must be determined by end user.

Kommentare

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Konzentration

Lot specific

Buffer

0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Konservierungsmittel

ProClin

Vorsichtsmaßnahmen

WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handhabung

Avoid repeated freeze-thaw cycles.

Lagerung

4 °C,-20 °C

Informationen zur Lagerung

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Haltbarkeit

24 months
Chen, Liu, Liu, Li, Cheng: "Transcription factor HBP1: A regulator of senescence and apoptosis of preadipocytes." in: Biochemical and biophysical research communications, Vol. 517, Issue 2, pp. 216-220, (2020) (PubMed).
Target

p53 (TP53) (Tumor Protein P53 (TP53))

Andere Bezeichnung

Tumor Protein p53 (TP53 Produkte)

Hintergrund

TP53, LFS1, TRP53, Li-Fraumeni Syndrome, Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53

Pathways

p53 Signalweg, MAPK Signalweg, PI3K-Akt Signalweg, Apoptose, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagie, Warburg Effekt