Urm1 Antikörper

Produktnummer ABIN108085

Hersteller Produkt- Nr.: 200-401-443

Produktdetails anti-Urm1 Antikörper

Target: Urm1 (Ubiquitin Related Modifier 1 (Urm1))

Reaktivität: Saccharomyces cerevisiae

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Urm1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Hersteller Produkt- Nr.: 200-401-443

Hersteller: Rockland

Verwendungszweck: Urm1 Antibody

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.

Produktmerkmale: Synonyms: rabbit anti-Urm1 Antibody, C9orf74 antibody, Chromosome 9 open reading frame 74 antibody, MGC2668 antibody, RP11 339B21.4 antibody, Ubiquitin Related Modifier 1 antibody, Ubiquitin related modifier 1 homolog antibody, Urm 1 antibody

Aufreinigung: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: This purified antibody was prepared from rabbit serum after repeated immunizations with recombinant yeast Urm1 protein.

Immunogen Type: Recombinant Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: This purified polyclonal antibody reacts with yeast Urm1 by western blot and ELISA. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation.   This antibody using the specified conditions may recognize other prominent intrinsic bands (UBLs or their conjugates).  Other intrinsic bands are readily detectable in yeast lysates at lower antibody dilutions. For immunoblotting a 12 kDa band corresponding to yeast Urm1 is detected.  Most yeast cell lysates can be used as a positive control without induction or stimulation. 

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:2,000 - 1:10,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 5.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Details zu Urm1

Target: Urm1 (Ubiquitin Related Modifier 1 (Urm1))

Andere Bezeichnung: URM1 (Urm1 Produkte)

Hintergrund: Background: Ubiquitin-like proteins fall into two classes: the first class, ubiquitin-like modifiers (UBLs) function as modifiers in a manner analogous to that of ubiquitin.  Examples of UBLs are SUMO, Rub1 (also called Nedd8), Apg8 and Apg12.  Proteins of the second class include parkin, RAD23 and DSK2, are designated ubiquitin-domain proteins (UDPs).  These proteins contain domains that are related to ubiquitin but are otherwise unrelated to each other.  In contrast to UBLs, UDPs are not conjugated to other proteins.  Urm1 is a newly identified ubiquitin related modifier.  Urm 1 is a 99-amino acid protein terminated with glycine-glycine.  Target proteins are conjugated to Urm1 via its C-terminal glycine. Initially Urm1 forms a thioester with a novel E1-like protein, Uba4.

Gen-ID: 854809

UniProt: P40554