VSV-g Tag Antikörper (AA 501-511)

Produktnummer ABIN108207

Hersteller Produkt- Nr.: 600-401-386

Produktdetails anti-VSV-g Tag Antikörper

Target: VSV-g Tag (VSV-G Epitope Tag)

Bindungsspezifität: AA 501-511

Reaktivität: Bitte anfragen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser VSV-g Tag Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Hersteller Produkt- Nr.: 600-401-386

Hersteller: Rockland

Verwendungszweck: VSV-G Antibody

Sequenz: YTDIEMNRLG K

Kreuzreaktivität (Details): This affinity purified antibody is directed against VSV-G protein.

Produktmerkmale: Synonyms: Rabbit Anti-VSV-G Epitope Tag Antibody, Rabbit Anti VSV-G Epitope Tag Antibody, Rabbit Anti-VSV-G Tag Antibody

Aufreinigung: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterilität: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 501-511 of vesicular stomatitis virus glycoprotein (VSV-G).

Immunogen Type: Conjugated Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:200 - 1:500

Application Note: This affinity purified antibody has been tested for use in ELISA and western blot. This product is suitable for immunofluorescence microscopy.  Specific conditions for reactivity should be optimized by the end user. For standard indirect immunofluorescence assay we recommend paraformaldehyde fixation with detergent permeabilization of cells infected with VSV or transfected with the wild-type VSV-G protein. Staining is quite clean and specific for both sources of the G protein when diluted at least 1/200.   At higher concentrations, a filamentous background staining may be present.

Western Blot Dilution: 1:1,000 - 1:5,000

ELISA Dilution: 1:50,000 - 1:100,000

IF Microscopy Dilution: 1:200 - 1:500

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.17 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen für anti-VSV-g Tag Antikörper (ABIN108207)

Muñoz-Álvarez, Altomonte, Laitinen, Ziegler, Steiger, Esposito, Schmid, Ebert: "PET imaging of oncolytic VSV expressing the mutant HSV-1 thymidine kinase transgene in a preclinical HCC rat model." in: Molecular therapy : the journal of the American Society of Gene Therapy, Vol. 23, Issue 4, pp. 728-36, (2016) (PubMed).

Details zu VSV-g Tag

Target: VSV-g Tag (VSV-G Epitope Tag)

Andere Bezeichnung: VSV-G (VSV-g Tag Produkte)

Substanzklasse: Tag

Hintergrund: Background: Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies.  Due to their small size, epitope tags do not affect the tagged protein's biochemical properties.   Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence.  This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means.  This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein.  The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures.  Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.  VSV-G or vesicular stomatitis virus glycoprotein is found within the pseudo lentiviral cloning vector pH CMV-VSV-G.