Aktivieren Sie Javascript in Ihrem Browser, um unsere Webseite optimal zu nutzen.

ACLY Antikörper (AA 306-502)

Name: ACLY Antikörper (AA 306-502)
Brand: antibodies-online
SKU: ABIN1098143
Price: 640.59 EUR
Availability: InStock

ACLY Reaktivität: Human WB, ELISA, IHC, FACS, ICC Wirt: Maus Monoclonal 5F8D11 unconjugated

Western blot analysis using ACLY mAb against human ACLY recombinant protein. (ACLY Antikörper (AA 306-502))

Flow cytometric analysis of HeLa cells using ACLY mouse mAb (green) and negative control (purple). (ACLY Antikörper (AA 306-502))

FACS

Immunofluorescence analysis of HeLa cells using ACLY mouse mAb (green). (ACLY Antikörper (AA 306-502))

Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues using ACLY mouse mAb with DAB staining. (ACLY Antikörper (AA 306-502))

IHC

Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using ACLY mouse mAb with DAB staining. (ACLY Antikörper (AA 306-502))

IHC

Western blot analysis using ACLY mouse mAb against HeLa (1), NIH3T3 (2), C6 (3), COS7 (4), and Raji (5) cell lysate. (ACLY Antikörper (AA 306-502))

Black line: Control Antigen (100 ng), Purple line: Antigen(10 ng), Blue line: Antigen (50 ng), Red line: Antigen (100 ng), (ACLY Antikörper (AA 306-502))

ELISA

7 Abbildungen

(2 Referenzen)

Produktnummer ABIN1098143

Datenblatt (PDF)

Target Alle ACLY Antikörper anzeigen

ACLY (ATP Citrate Lyase (ACLY))
Bindungsspezifität
19

15

6

5

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 306-502
Reaktivität
92

31

30

8

6

5

4

3

3

3

3

2

2

2

1

1
Human
Wirt
82

12
Maus
Passende Sekundärantikörper
Klonalität
71

23
Monoklonal
Konjugat
48

5

4

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2
Dieser ACLY Antikörper ist unkonjugiert
Applikation
76

33

27

26

14

13

13

13

7

6

4

3

3

2
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Verwendungszweck

ACLY Antibody

Aufreinigung

Purified antibody

Immunogen

Purified recombinant fragment of human ACLY (AA: 306-502 ) expressed in E. Coli.

Klon

5F8D11

Isotyp

IgG1
anti-ATP Citrate Lyase (ACLY) (AA 752-1101) antibody
ACLY Reaktivität: Human WB, IHC, IF Wirt: Kaninchen Polyclonal unconjugated

anti-ATP Citrate Lyase (ACLY) (pSer455) antibody
ACLY Reaktivität: Human WB, IHC, IP Wirt: Kaninchen Polyclonal unconjugated

anti-ATP Citrate Lyase (ACLY) (AA 306-502) antibody
ACLY Reaktivität: Human, Maus, Ratte, Affe WB, ELISA, IHC, FACS, IF/ICC Wirt: Maus Monoclonal unconjugated

anti-ATP Citrate Lyase (ACLY) (AA 4-265) antibody
ACLY Reaktivität: Human WB, ELISA, IHC, IF Wirt: Kaninchen Polyclonal unconjugated

anti-ATP Citrate Lyase (ACLY) antibody
ACLY Reaktivität: Human WB, ELISA, IF, FACS Wirt: Kaninchen Monoclonal 3A5 unconjugated Recombinant Antibody

anti-ATP Citrate Lyase (ACLY) (C-Term) antibody
ACLY Reaktivität: Human, Maus WB, FACS, ICC Wirt: Maus Monoclonal unconjugated

anti-ATP Citrate Lyase (ACLY) (Middle Region) antibody
ACLY Reaktivität: Human, Maus, Ratte, Rind (Kuh), Schwein, Zebrafisch (Danio rerio), Kaninchen, Schaf, Hund, Pferd WB, IHC Wirt: Kaninchen Polyclonal unconjugated

anti-ATP Citrate Lyase (ACLY) (Ser1227) antibody
ACLY Reaktivität: Human, Maus, Ratte, Affe WB, ELISA, IF Wirt: Kaninchen Polyclonal unconjugated

anti-ATP Citrate Lyase (ACLY) antibody
ACLY Reaktivität: Human, Maus, Ratte ELISA, IHC Wirt: Kaninchen Polyclonal unconjugated

anti-ATP Citrate Lyase (ACLY) (AA 793-1008) antibody
ACLY Reaktivität: Human WB, IF, ICC Wirt: Kaninchen Polyclonal unconjugated

Applikationshinweise

ELISA: 1/10000
FCM: 1/200 - 1/400
ICC: 1/50

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Buffer

Purified antibody in PBS with 0.05 % sodium azide.

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

4 °C,-20 °C

Informationen zur Lagerung

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Chu, Lin, Hendel, Kulpa, Brownsey, Johnson: "ATP-citrate lyase reduction mediates palmitate-induced apoptosis in pancreatic beta cells." in: The Journal of biological chemistry, Vol. 285, Issue 42, pp. 32606-15, (2010) (PubMed).

Beckner, Fellows-Mayle, Zhang, Agostino, Kant, Day, Pollack: "Identification of ATP citrate lyase as a positive regulator of glycolytic function in glioblastomas." in: International journal of cancer. Journal international du cancer, Vol. 126, Issue 10, pp. 2282-95, (2010) (PubMed).
Target

ACLY (ATP Citrate Lyase (ACLY))

Andere Bezeichnung

ACLY (ACLY Produkte)

Hintergrund

Description: ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Two transcript variants encoding distinct isoforms have been identified for this gene.

Aliases: ACL, ATPCL, CLATP

Molekulargewicht

125kDa

Gen-ID

47

HGNC

47

UniProt

P53396

Pathways

Warburg Effekt