Human Complement C3c. In immunoelectrophoresis against fresh human serum, a single precipitin line is obtained in the beta-1 region representing native C3. Against serum containing partly activated C3, a precipitin line is obtained which extends from the beta-1 into the alpha-2 region, demonstrating a gradient. In old serum containing totally activated C3 a single precipitin line in the alpha-2 region is obtained. Antisera to C3c can also react with the fragments C3b, C3bi and smaller fragments, since they all carry antigenic determinants of the C3c domain. The product does not react with any other proteins component of human serum or plasma. Cross-reactivity Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
C3C
Reaktivität: Maus
ELISA, IHC, IF
Wirt: Ziege
Polyclonal
unconjugated
Applikationshinweise
Approved: ID, IHC-P (1:200)
Usage: This product is intended for use in precipitating techniques as immunoelectrophoresis and single and double radial immunodiffusion (Mancini, Ouchterlony) to identify the presence of complement C3c or to determine its concentration. The presence of non-precipitating antibodies has not been assayed. This does not exclude the use of the antiserum in non-precipitating antibody-binding techniques if proper controls are included. Determinations of individual complement components can be very useful in defining the exact location of a defect.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Sterile distilled water 1 ml
Konzentration
Lot specific
Buffer
Lyophilized antiserum
Handhabung
Avoid freeze/thaw cycles. Store undiluted.
Lagerung
4 °C,-20 °C
Informationen zur Lagerung
Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze/thaw cycles. Store undiluted.