Follistatin Antikörper

Produktnummer ABIN1101625

Produktdetails anti-Follistatin Antikörper

Target: Follistatin (FST)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Follistatin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Neutralization (Neut)

Marke: IHC-plus™

Spezifität: Human Follistatin

Aufreinigung: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human Follistatin.

Type of Immunogen: Cell extract

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (0.5 - 2 μg/mL), IHC, IHC-P (5 μg/mL), Neut (8 - 12 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Immunohistochemistry: LS-B7434 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 μg/mL is recommended. Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human Follistatin (100 ng/mL), a concentration of 8-12 μg/mL of this antibody is required. ELISA: To detect Human Follistatin by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human Follistatin (LS-C104465) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human Follistatin. Western Blot: To detect Human Follistatin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human Follistatin is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Sterile water

Konzentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handhabung: Avoid freeze/thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Details zu Follistatin

Target: Follistatin (FST)

Andere Bezeichnung: FST / Follistatin (FST Produkte)

Hintergrund: Name/Gene ID: FST

Synonyms: FST, Activin-binding protein, Follistatin isoform FST317, Follistatin, Fs

Gen-ID: 10468

UniProt: P19883

Pathways: Negative Regulation of Hormone Secretion