VEGFA Antikörper

Produktnummer ABIN1104981

Produktdetails anti-VEGFA Antikörper

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser VEGFA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Spezifität: This purified antibody has been heated to 56 °C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human VEGF-165 in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.

Aufreinigung: Ion exchange chromatography

Immunogen: This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human VEGF-165 protein.

Type of Immunogen: Recombinant protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, WB (1:1000)

Usage: This purified antibody has been tested for use in ELISA and western blotting. Reactivity is also expected in neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be <10 pg/ul by the LAL method. By western blot a band approximately 22 kD in size corresponding to native monomeric human VEGF-165 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: deionized water

Konzentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2

Handhabung: avoid freeze thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Details zu VEGFA

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Andere Bezeichnung: VEGFA / VEGF (VEGFA Produkte)

Hintergrund: Name/Gene ID: VEGFA
Family: PDGF

Synonyms: VEGFA, VPF, Vascular permeability factor, VEGF, VEGF-A, MVCD1

Gen-ID: 7422

UniProt: P15692

Pathways: RTK Signalweg, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling