E. Coli Antikörper

Produktnummer ABIN110776

Produktdetails anti-E. Coli Antikörper

Target: E. Coli

Reaktivität: E. coli

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser E. Coli Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Western Blotting (WB), Enzyme Immunoassay (EIA)

Aufreinigung: Affinity Chromatography on Protein A

Immunogen: Escherichia coli J5 cells.

Klon: 2D7-1

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Immunofluorescence. ELISA: (a 1 mg/mL solution has been used at dilutions of up to 1/1500).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: PBS, pH 7.2, 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu E. Coli

Target: E. Coli

Abstract: E. Coli Produkte

Substanzklasse: Bacteria

Hintergrund: Some form of LPS is a major component of the cell membrane of Gram negative bacteria, contributing greatly to the structural integrity of the bacteria, and protecting the membrane from certain kinds of chemical attack. LPS is an endotoxin, and induces a strong response from normal animal immune systems. LPS function has been under experimental research for several years due to its role in activating many transcriptional factors, which become active after stimulation with LPS. LPS also produces many types of mediators involved in septic shock. The J5 mutant of E. coli lacks the enzyme uridine diphosphate glucose 4-epimerase and therefore produces an incomplete LPS, deficient in galactose and all the sugars distal to the central polymers. The 55 mutant has no O-specific chains and its endotoxin remains as the core LPS containing lipid A, N acetyl glucosamine, 2-keto-3-deoxyoctonate, heptose and glucose (a composition similar to that of the Rc strains of Salmonella).Synonyms: EHEC, EPEC, ETEC, Escherichia coli