Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))
Spezifität
This Monoclonal antibody clone 1011 is specific for a 104- kDa protein. This mAb is one of four mAb which recognize different epitopes of the protein. Cells whose supernatant showed a positive signal for a 104- kDa band were selected and cloned by limited dilution.
Kreuzreaktivität (Details)
Species reactivity (tested):Human.
Aufreinigung
Purified
Immunogen
A BALB/c mouse was immunized with 5 myg of affinity purified nuclear extract proteins. Spleen cells were fused with equal number of Sp0Ag-14 myeloma cells.
LRP antikoerper, VAULT1 antikoerper, 2310009M24Rik antikoerper, cb771 antikoerper, wu:fb52b05 antikoerper, wu:fc02g01 antikoerper, mvp antikoerper, MGC145641 antikoerper, Tb05.45E22.810 antikoerper, major vault protein antikoerper, major vault protein L homeolog antikoerper, putative major vault protein antikoerper, MVP antikoerper, Mvp antikoerper, mvp antikoerper, mvp.L antikoerper, Tc00.1047053510353.10 antikoerper, Tb927.5.4460 antikoerper, Tb10.70.0520 antikoerper, Tb10.70.5840 antikoerper, LMJF_05_0060 antikoerper
Hintergrund
MVP is identical to lung-resistance related protein (LRP). Vaults are large ribonucleoprotein particles (RNPs) present in all eukaryotic cells. They have a complex morphology, including several small molecules of RNA, but a single protein species. The MVP accounts for >70 % of their mass. Their shape is reminiscent of the nucleopore central plug. Amino acid 241-280 of human estrogen receptor (ER), (site of nuclear localization signal sequence), is mapped to be the site of interaction between ER and MVP. Treatment of cells with estradiol increases the amount of MVP in nuclear extract. Anti-estrogen 1C1182 shows no effect. The hormone-dependent interaction of vaults with ER is prevented in vitro by sodium molybdate. Antibodies to progesterone and glucocorticoid receptors are also able to co-immunoprecipitate the MVP. LRP is a protein overexpressed in many neoplastic tissues and cell lines. Expression of LRP predicts a poor response to chemotherapy.This 104-kD protein is the major vault protein (MVP) also described as the lung resistance protein (LRP) and has shown to interact with the estrogen receptor. The protein is part of a very large vault ribonucleoprotein complex present in all eukaryotic cells and its structure and protein composition is highly conserved. Because of the size, shape, and protein and RNA composition of this complex the particles are different from other ribonucleoproteins.Synonyms: LRP, Lung-Resistance Related Protein, MVP