Topoisomerase II alpha Antikörper (N-Term)

Produktnummer ABIN1109282

Produktdetails anti-Topoisomerase II alpha Antikörper

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Bindungsspezifität: N-Term

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Topoisomerase II alpha Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Spezifität: This antibody reacts with Topo IIa and ß. The antibody detects 170 KD of human Topo IIa and 180 KD of human Topo IIß on Western blot with whole cell lysate.

Kreuzreaktivität (Details): Species reactivity (tested):Human (Jurkat, Raji), Mouse (NIH3T3, P19, WR19L), Rat (PC12)

Aufreinigung: Protein-A Sepharose

Immunogen: Recombinant N-terminal Topoisomerase II fusion protein

Klon: AK5

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS containing 50 % glycerol, pH 7.2, without preservatives

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store (in aliquots) at -20 °C.

Details zu Topoisomerase II alpha

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Andere Bezeichnung: TOP2A (TOP2A Produkte)

Synonyme: TOP2 antikoerper, TP2A antikoerper, TOP2A antikoerper, Top-2 antikoerper, TOP-2 antikoerper, F12B17.110 antikoerper, F12B17_110 antikoerper, thimet metalloendopeptidase 2 antikoerper, fc08c07 antikoerper, wu:fc08c07 antikoerper, DNA topoisomerase II alpha antikoerper, DNA topoisomerase II, alpha isozyme antikoerper, topoisomerase (DNA) II alpha antikoerper, Zincin-like metalloproteases family protein antikoerper, TOP2A antikoerper, Bm1_08705 antikoerper, Top2a antikoerper, AT5G10540 antikoerper, top2a antikoerper

Hintergrund: Eukaryotic DNA Topoisomerase II is a ubiquitous ATP-dependent type II Topoisomerase that play an important role in DNA replication, transcription and recombination by changing the degree of superhelicity, catenation and knotting of topologically closed DNA molecules. Two isoforms of DNA Topoisomerases II have been reported in mammalian cells, Topoisomerase IIa and IIß. cDNA sequence of those isoforms reveals that both type II Topoisomerases are structurally and catalytically close. However, Topoisomerase IIß is expressed with no cell cycle dependence, whereas the expression of Topoisomerase IIa is cell cycle-regulated, peaking in G2-M phase and declining to a minimal level at the end of M phase. Topoisomerase IIa is poly-ubiquitinated and degraded preferentially in the apoptosis-induced human epithermoid carcinoma-derived cell line, MA1, in an ATP-and-ubiquitin-dependent manner. Recent studies also indicated that this isoform is selectively cleaved during Fas-mediated T cell apoptosis and Fas-, Staurosporine- and Anisomycin-mediated Jurkat cell apoptosis.Synonyms: DNA topoisomerase 2-alpha, DNA topoisomerase 2-beta, DNA topoisomerase II alpha isozyme, DNA topoisomerase II beta isozyme, TOP2, Top-2

Gen-ID: 7153

NCBI Accession: NP_001058

UniProt: P11388

Pathways: Zellzyklus, Mitotic G1-G1/S Phases