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Vimentin Antikörper (pSer55)

Name: Vimentin Antikörper (pSer55)
SKU: ABIN1109485
Price: 759.23 EUR
Availability: InStock

VIM Reaktivität: Human, Maus, Ratte WB, IF Wirt: Maus Monoclonal 4A4 unconjugated

Western Blotting (WB) image for anti-Vimentin (VIM) (pSer55) antibody (ABIN1109485) (Vimentin Antikörper (pSer55))

Immunofluorescence (IF) image for anti-Vimentin (VIM) (pSer55) antibody (ABIN1109485) (Vimentin Antikörper (pSer55))

2 Abbildungen

Produktnummer ABIN1109485

Datenblatt (PDF)

Target Alle Vimentin (VIM) Antikörper anzeigen

Vimentin (VIM)
Bindungsspezifität
32

16

16

15

11

9

9

7

4

3

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
pSer55
Reaktivität
305

120

117

61

60

50

43

38

30

22

20

14

13

8

8

6

6

3

3

2

1

1

1

1

1

1

1

1

1
Human, Maus, Ratte
Wirt
158

130

11

6

6

5

2
Maus
Passende Sekundärantikörper
Klonalität
172

145
Monoklonal
Konjugat
198

15

13

12

9

7

3

3

3

3

3

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1
Dieser Vimentin Antikörper ist unkonjugiert
Applikation
263

115

105

97

88

64

48

40

39

33

23

20

8

5

4

4

3

2

2

2

1

1

1

1

1

1

1

1

1
Western Blotting (WB), Immunofluorescence (IF)

Sequenz

SLYSS-phosphoS55-PGGAYC-KLH

Spezifität

This antibody reacts specifically with the phosphorylated MPV55 peptide but not the non-phosphorylated peptide. This antibody detects vimentin phosphorylated by cdc2 kinase and does not detect non-phosphorylated vimentin or phosphorylated vimentin by cAMP-dependent kinase, protein kinase C, or Ca2+-calmodulin-dependent protein kinase II on Western blotting.

Kreuzreaktivität (Details)

Species reactivity (tested):Human, mouse, rat

Aufreinigung

Protein A agarose

Immunogen

Synthetic MPV55 phosphopeptide corresponding to mouse phosphorylated vimentin Ser55.

Klon

4A4

Isotyp

IgG2b

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VIM Reaktivität: Human, Maus, Ratte, Schwein, Huhn WB, IHC (p), ICC, IP Wirt: Maus Monoclonal VI-10 unconjugated

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VIM Reaktivität: Human, Maus WB, IF, FACS, IHC (p) Wirt: Kaninchen Polyclonal RB41034 unconjugated

anti-Vimentin (VIM) (AA 1-200) antibody
VIM Reaktivität: Human WB, IHC, IF Wirt: Kaninchen Polyclonal unconjugated

anti-Vimentin (VIM) antibody
VIM Reaktivität: Human WB, IHC, ICC, IP Wirt: Maus Monoclonal C8 unconjugated

anti-Vimentin (VIM) (AA 430-457), (C-Term) antibody
VIM Reaktivität: Human WB, IF, FACS, IHC (p) Wirt: Kaninchen Polyclonal RB16691 unconjugated

anti-Vimentin (VIM) (AA 41-140) antibody
VIM Reaktivität: Human WB, ELISA, IF, IP Wirt: Maus Monoclonal 3E9 unconjugated

anti-Vimentin (VIM) (AA 2-466) antibody
VIM Reaktivität: Human WB, IHC, ELISA, FACS Wirt: Maus Monoclonal 3A1F2 unconjugated

Applikationshinweise

Optimal working dilution should be determined by the investigator.

Beschränkungen

Nur für Forschungszwecke einsetzbar
Konzentration

1.0 mg/mL

Buffer

PBS containing 50 % glycerol, pH 7.2. No preservative is contained.

Konservierungsmittel

Azide free

Handhabung

Avoid repeated freezing and thawing.

Lagerung

-20 °C

Informationen zur Lagerung

Store (in aliquots) at -20 °C.
Target

Vimentin (VIM)

Andere Bezeichnung

Vimentin (VIM Produkte)

Hintergrund

Vimentin is an intermediate filament protein distributed widely in the cytoplasm and is phosphorylated by several protein kinase in vitro. Ser55 residues on vimentin were reported to be one of the phosphorylation sites of vimentin at metaphase and were the phosphorylation sites for cdc2 kinase but not for cAMP-dependent protein kinase, protein kinase C, and Ca2+-calmodulin-dependent protein kinase II in vitro. Immunofluorescence and immunoelectron microscopy showed that vimentin Ser55 residues distributed in the entire cytoplasmic vimentin filament system are phosphorylated when the cells enter mitosis and de-phosphorylated in cytokinesis. The use of this antibody that specifically reacts with the phosphorylation site of vimentin Ser55 by cdc2 kinase enables estimation of a particular cdc2 kinase function.Synonyms: VIM

Gen-ID

7431

NCBI Accession

NP_003371

UniProt

P08670

Pathways

Caspase Kaskade in der Apoptose